摘要
Multicellular spheroids,which mimic the natural organ counterparts,allow the prospect of drug screening and regenerative medicine.However,their application is hampered by low processing efficiency or limited scale.This study introduces an efficient method to drive rapid multicellular spheroid formation by a cellulose nanofibril matrix.This matrix enables the facilitated growth of spheroids(within 48 h)through multiple cell assembly into size-controllable aggregates with well-organized physiological microstructure.The efficiency,dimension,and conformation of the as-formed spheroids depend on the concentration of extracellular nanofibrils,the number of assembled cells,and the heterogeneity of cell types.The above strategy allows the robust formation mechanism of compacted tumoroids and hepatocyte spheroids.
基金
supported by the National Natural Science Foundation of China(No.32071347)
the ZJU-Hangzhou Global Scientific and Technological Innovation Center,Zhejiang University(No.02020200-K02013008)
the Joint Laboratory Grant from Jiangsu Wuzhong Aesthetics Biotech Co.,Ltd.,and the Starting Grant of ShanghaiTech University.
