摘要
目的采用电感耦合等离子体串联质谱技术建立人体尿液中钛(Ti)元素的快速检测方法。方法比较不同测定模式,选择47Ti做为测定同位素元素。在碰撞/反应池中通入氨气,通过质谱全扫确定质谱条件一级四极杆质量过滤器质量数Q1=47,二级四极杆质量过滤器质量数Q2=149(钛氨六聚体)。通过优化内标元素质谱条件、积分时间、反应气流量以及数据处理方式等确定最佳条件参数。结果Ti在0.1~5.00μg/L范围内线性关系良好,检出限为0.1μg/L,日内精密度和日间精密度均小于10%,平均回收率为93.7%~112.2%,Seronorm Trace Elements Urine L-2冻干尿标准参考物质的测定结果在标准物质证书可接受范围内。结论该方法操作快速、简便、测定结果准确,适合人体尿液中钛元素的快速分析。
OBJECTIVE To establish an inductively coupled plasma-tandem mass spectrometry(ICP-MS/MS)method to determinate the titanium(Ti)in human urine samples rapidly.METHODS By comparing different gas mode,~(47)Ti was selected as the analysis element.Injecting NH_3 to the collision/reaction cell,swept by mass spectrum to determine the optimal mass spectrum conditions with the mass number of Q1=47,Q2=149(Ti ammonia hexamer).The mass spectrometry conditions of internal standard elements,integration time,reaction gas flow rate and data processing method and so on were optimized.RESULTS Ti had a good linear between 0.1-5.00μg/L under the optimal analytical conditions.The detection limit was 0.1μg/L.The RSDs of intra-day and inter-day were less than 10%,and the average recoveries were 93.7%-112.2%.The result of Seronorm Trace Elements Urine L-2 met the acceptable range.CONCLUSION The method is rapid,simple,accuracy,and suitable for the rapid analysis of titanium in human urine.
作者
张淼
赵志飞
丁亮
Zhang Miao;Zhao Zhifei;Ding Liang(National Institute of Environmental Health,Chinese Center for Disease Control and Prevention,Beijing 100021,China;Agilent Technologies(China)Co.,Ltd,Beijing 100102,China)
出处
《卫生研究》
CAS
CSCD
北大核心
2023年第4期604-610,共7页
Journal of Hygiene Research
关键词
钛
尿
电感耦合等离子体串联质谱
titanium
urine
inductively coupled plasma-tandem mass spectrometry
