摘要
为明确水草环境对克氏原螯虾(Procambarus clarkii)生长发育的影响机制,采用生态试验学方法,以轮叶黑藻(Hydrilla verticillata)和水花生(Alternanthera philoxeroides)及平均体质量为(3.54±0.73)g的幼虾为研究对象,设置对照组(仿生水草,BM)、轮叶黑藻组(HM)和水花生组(AM)进行为期62 d的养殖试验。结果表明:养殖试验结束后,HM组克氏原螯虾的含肉率显著高于BM组(P<0.05),HM和AM组的成活率和总质量均高于BM组(P>0.05),HM组分别较BM组提高12.12%、11.42%,AM组分别较BM组提高16.68%、25.78%;HM和AM组肝胰腺胰蛋白酶和纤维素酶活性显著高于BM组(P<0.05),两组肠道胰蛋白酶和纤维素酶活性则显著低于BM组(P<0.05);各组氨基酸含量无明显差异(P>0.05);各组脂肪酸含量差异较大,HM和AM组肌肉∑(n-3)PUFA/∑(n-6)PUFA显著高于BM组(P<0.05),HM组肌肉EPA、DHA、∑SFA和∑PUFA含量均显著高于BM组(P<0.05)。研究表明,克氏原螯虾养殖过程中,水草环境对克氏原螯虾的生长性能、生理指标及肌肉营养品质存在显著影响,种植轮叶黑藻更有利于提高克氏原螯虾的肌肉品质。
In order to clarify influence mechanism of aquatic plant environment on growth and development of red swamp crayfish(Procambarus clarkii),juvenile red swamp crayfish with body weight of(3.54±0.73)g were reared in a 2.5 m length×2.0 m wide×1.2 m high canvas box with 0.3 m depth of soil at bottom for planting aquatic plants Hydrilla verticillata(group HM),Alternanthera philoxeroides(group AM)and bionic plastic aquatic plant(control group,BM)at coverage rate of 50%at initial density of 30 crayfish per box at water temperature of(23.51±2.92)℃for 62 d.Breeding period,the culture boxes were aerated for oxygenation at night and on rainy days.The results showed that there was significantly higher dressing rate in the crayfish in group HM than that in the BM group(P<0.05).The crayfish had higher survival rate and total weight in groups HM and AM than those in the control group did,increased by 12.12%,11.42%and 16.68%,25.78%,respectively.The activities of trypsin and cellulose in hepatopancreas were shown to be significantly higher in groups HM and AM than those in group BM(P<0.05),and significantly lower activities of trypsin and cellulase in intestine in groups HM and AM than those in group BM(P<0.05),without significant difference in amino acid content among all groups(P<0.05).∑(n-3)polyunsaturated fatty acid(PUFA)/∑(n-6)PUFA of muscle was significantly higher in HM and AM groups than that in group BM(P<0.05).The contents of EPA,DHA,∑SFA and∑PUFA were found to be significantly higher in the muscle in group HM than those in group BM(P<0.05).The findings indicate that the aquatic vegetation environment has a significant impact on the growth performance,physiological indicators,and muscle nutrient quality of red swamp crayfish during cultivation.Planting H.verticillata is more conducive to improving the muscle quality of crayfish.
作者
吴雷明
韩光明
覃宝利
张家宏
王守红
寇祥明
袁秦
毕建花
唐鹤军
WU Leiming;HAN Guangming;QIN Baoli;ZHANG Jiahong;WANG Shouhong;KOU Xiangming;YUAN Qin;BI Jianhua;TANG Hejun(Lixiahe District Agricultural Institute of Jiangsu,Yangzhou 225007,China;Ecological Agricultural Engineering Technology Research Center of Jiangsu,Yangzhou 225009,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2023年第5期779-786,共8页
Journal of Dalian Ocean University
基金
江苏省重点研发计划项目(BE2021336)
江苏省农业自主创新资金资助项目[CX(21)1008]
江苏省现代农业产业技术体系(JATS[2022]265,JATS[2022]268)
扬州市重点研发计划项目(YZ2022038)
江苏里下河地区农业科学研究所基金资助项目[SJ(22)205,SJ(22)110]。
关键词
轮叶黑藻
水花生
生长性能
氨基酸
脂肪酸
Hydrilla verticillate
Alternanthera philoxeroides
growth performance
amino acid
fatty acid
