摘要
目的探究血清长链非编码RNA(lncRNA)结肠癌相关转录因子1(CCAT1)对非酒精性脂肪性肝病(NAFLD)患者肝纤维化及其程度的诊断价值。方法选择2018年2月至2021年12月在树兰(杭州)医院住院治疗的256例NAFLD患者作为研究对象,按照有无肝纤维化将其分为肝纤维化组(156例)和无肝纤维化组(100例),将肝纤维化组患者按照肝纤维化程度分为轻中度肝纤维化组(112例)和重度肝纤维化组(44例)。收集患者的临床资料,采用实时荧光定量PCR法检测各组的血清lncRNA CCAT1(以下简称为CCAT1)水平并比较组间差异;采用Pearson相关性分析法探讨血清CCAT1与肝纤维化指标的相关性;采用ROC曲线分析血清CCAT1对NAFLD患者肝纤维化及其程度的诊断价值。结果肝纤维化组的血清CCAT1水平显著高于无肝纤维化组(P<0.05),重度肝纤维化组的血清CCAT1水平显著高于轻中度肝纤维化组(P<0.05)。血清CCAT1与肝纤维化指标Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(CⅣ)、层粘连蛋白(LN)均呈正相关(P均<0.05)。血清CCAT1诊断NAFLD患者肝纤维化的ROC曲线下面积(AUC)为0.742,最佳截断值为1.37,敏感度和特异度分别为55.57%和83.00%。血清CCAT1诊断NAFLD患者重度肝纤维化的AUC为0.795,最佳截断值为1.43,敏感度和特异度分别为84.09%和63.39%。结论NAFLD患者中,有肝纤维化患者的血清CCAT1水平高于无肝纤维化患者,且血清CCAT1水平随肝纤维化程度的加重而升高。血清CCAT1对于NAFLD患者肝纤维化及其程度的诊断具有一定价值。
Objective An attempt is made in this paper to exploring the diagnostic value of serum long chain non coding RNA(lncRNA)and colon cancer related transcription factor 1(CCAT1)for liver fibrosis and its degree in patients with non-alcoholic fatty liver disease(NAFLD).Methods Two hundreds and fifty-six NAFLD patients who were hospitalized at Shulan(Hangzhou)Hospital from February 2018 to December 2021 were selected and assigned into the liver fibrosis group(156 cases)and the non liver fibrosis group(100 cases)based on the presence or absence of liver fibrosis.The patients in the liver fibrosis group were divided into the mild to moderate liver fibrosis group(112 cases)and the severe liver fibrosis group(44 cases)according to the degree of liver fibrosis.The clinical data of patients were collected,and the realtime fluorescence quantitative PCR was used to detect the serum lncRNA CCAT1(Hereinafter referred to as CCAT1)levels in each group and compare the differences between the groups.The Pearson correlation analysis was used to explore the correlation between serum CCAT1 and liver fibrosis indicators,while the ROC curve was used to analyze the diagnostic value of serum CCAT1 for liver fibrosis and its degree in NAFLD patients.Results The serum CCAT1 level in the liver fibrosis group is significantly higher than that in the non liver fibrosis group(P<0.05),while that in the severe liver fibrosis group is significantly higher than that in the mild to moderate liver fibrosis group(P<0.05).The serum CCAT1 is positively correlated with the liver fibrosis indicators such as typeⅢprocollagen(PCⅢ),typeⅣcollagen(CⅣ),and laminin(LN)(P<0.05).The area under the ROC curve(AUC)of serum CCAT1 in diagnosing liver fibrosis in NAFLD patients is 0.742,with an optimal cutoff value of 1.37,and a sensitivity and specificity of 55.57%and 83.00%,respectively.The AUC of serum CCAT1 in diagnosing severe liver fibrosis in NAFLD patients is 0.795,with an optimal cutoff value of 1.43,and a sensitivity and specificity of 84.09%and 63.39%,
作者
谷娟娟
丁茹
周晓娜
周昌虎
GU Juanjuan;DING Ru;ZHOU Xiaona;ZHOU Changhu(Department of Gastroenterology,Shulan(Hangzhou)Hospital,Hangzhou 310015,China;Department of Gastroenterology,Hangzhou Danqing Jiangnan Hospital,Hangzhou 311421,China)
出处
《国际消化病杂志》
CAS
2023年第5期317-322,共6页
International Journal of Digestive Diseases
基金
浙江省医药卫生科技计划项目(2023KY218)。
关键词
非酒精性脂肪性肝病
结肠癌相关转录因子1
肝纤维化
Non-alcoholic fatty liver disease
Colon cancer related transcription factor 1
Liver fibrosis
