摘要
目的分析错配修复蛋白(MSH2、MSH6、MLH1、PMS2)以及人表皮生长因子受体2(Her-2)表达与结直肠癌患者淋巴结转移的相关性。方法选择2020年12月—2023年1月东营市河口区中医院收治的116例结直肠癌患者作为研究对象,采用免疫组化法检测错配修复蛋白MSH2、MSH6、MLH1、PMS2表达和组织中Her-2表达阳性率。根据结直肠癌患者是否发生淋巴结转移分为淋巴结转移组和无淋巴结转移组,比较2组错配修复蛋白MSH2、MSH6、MLH1、PMS2表达及Her-2表达阳性率;采用Logistic回归分析法分析上述蛋白表达阳性与结直肠癌患者淋巴结转移的相关性;绘制受试者工作特征曲线(ROC曲线)并计算ROC曲线下面积(AUC),考察错配修复蛋白及Her-2表达阳性对结直肠癌淋巴结转移的评估价值。结果病理学诊断结果显示,116例结直肠癌患者中有淋巴结转移59例,无淋巴结转移57例。淋巴结转移组和无淋巴结转移组的MSH6表达阳性率比较差异无统计学意义(93.22%比96.49%,P>0.05);淋巴结转移组的MSH2、MLH1、PMS2缺失率及Her-2阳性表达率均明显高于无淋巴结转移组,差异均有统计学意义(MSH2:15.25%比1.75%,MLH:16.95%比3.51%,PMS2:15.25%比1.75%,Her-2:47.46%比24.56%,均P<0.05)。Logistic回归分析结果显示,错配修复蛋白MSH6表达阳性与结直肠癌患者淋巴转移无相关性〔优势比(OR)为2.000,95%可信区间(95%CI)为0.352~11.372〕,错配修复蛋白MSH2、MLH1、PMS2表达及Her-2表达阳性与结直肠癌患者淋巴转移呈正相关(OR值分别为10.080、5.612、10.080、2.774,95%CI为1.233~82.388、1.172~26.875、1.233~82.388、1.258~6.116)。ROC曲线分析显示,错配修复蛋白MSH2、MSH6、MLH1、PMS2表达及Her-2表达评估结直肠癌患者淋巴转移的AUC分别为0.567、0.516、0.567、0.567、0.614,均有一定评估价值,但评估性能不高。结论错配修复蛋白MSH2、MSH6、MLH1、PMS2表达缺失及Her-2阳性表达均与结直肠癌患者淋巴结转移�
Objective To analyze the correlation between the expression of mismatch repair proteins(MSH2,MSH6,MLH1 and PMS2)and human epidermal growth factor receptor 2(Her-2)with lymph node metastasis in colorectal cancer patients.Methods The 116 patients with colorectal cancer admitted to Hekou District Traditional Chinese Medicine Hospital of Dongying City from December 2020 to January 2023 were selected as research objects.Immunohistochemical method was used to detect the expression positive rates of mismatch repair proteins MSH2,MSH6,MLH1,PMS2,and Her-2 in tissues.According to whether colorectal cancer patients had lymph node metastasis,they were divided into lymph node metastasis group and non-lymph node metastasis group.The expression positive rate of mismatch repair proteins MSH2,MSH6,MLH1,PMS2,and Her-2 were compared between the two groups.Logistic regression analysis was used to analyze the correlation between positive protein expression and lymph node metastasis in colorectal cancer patients.The reciever operator characteristic(ROC)curve was drawn and the area under ROC curve(AUC)was calculated to investigate the evaluation value of positive expression of mismatch repair protein and Her-2 in lymph node metastasis.Results The pathological diagnosis results showed that out of 116 patients with colorectal cancer,59 cases had lymph node metastasis and 57 cases had no lymph node metastasis.There was no significant difference in the positive rate of MSH6 expression between lymph node metastasis group and non-lymph node metastasis group(93.22%vs.96.49%,P>0.05).The deletion rates of MSH2,MLH1,PMS2 and the positive expression rate of Her-2 in lymph node metastasis group were significantly higher than those in non-lymph node metastasis group,with statistical significant differences(MSH2:15.25%vs.1.75%,MLH:16.95%vs.3.51%,PMS2:15.25%vs.1.75%,Her-2:47.46%vs.24.56%,all P<0.05).The results of Logistic regression analysis showed that there was no correlation between the positive expression of mismatch repair protein MSH6 and lymp
作者
贾红梅
Jia Hongmei(Department of Clinical Laboratory,Hekou District Traditional Chinese Medicine Hospital of Dongying City,Dongying 257234,Shandong,China)
出处
《实用检验医师杂志》
2023年第3期320-324,共5页
Chinese Journal of Clinical Pathologist
关键词
结直肠癌
淋巴结转移
错配修复蛋白
人表皮生长因子受体2
Colorectal cancer
Lymphatic metastasis
Mismatch repair protein
Human epidermal growth factor receptor 2
