摘要
目的建立芪风通窍合剂中毛蕊异黄酮葡萄糖苷的含量测定方法。方法采用高效液相色谱法测定芪风通窍中毛蕊异黄酮葡萄糖苷的含量,色谱柱为phenomenex Kinetex■EVO C_(18)(4.6 mm×250 mm,5μm,100 A);流动相为乙腈-0.2%冰乙酸(13∶87);检测波长260 nm;流速1 mL·min^(-1),柱温25℃。结果毛蕊异黄酮葡萄糖苷在40.85~816.50 ng范围内,进样量与峰面积呈良好线性关系(r=0.9994),平均回收率为102.02%,RSD为3.18%。结论本方法灵敏度高、专属性强、重复性好,可用于芪风通窍合剂中毛蕊异黄酮葡萄糖苷的含量测定。
OBJECTIVE To establish the method for determing the content of calycosin-7-glucoside in Qifengtongqiao mixture.METHODS The high performance liquid chromatography(HPLC)was used to determine the content of calycosin-7-glucoside which acquired with phenomenex Kinetex■EVO C_(18)(4.6 mm×250 mm,5μm,100 A)at a flow rate of 1.0 mL·min^(-1)with a mobile phase consisting of acetonitrile-0.2%ice acetic acid(13∶87,v/v).The detection wavelength was 260 nm and the column temperature was 25℃.RESULTS The concentration of calycosin-7-glucoside in 40.85-816.50 ng showed a good linear relationship between injection volume and peak area,and the correlation coefficient was 0.9994.The average recovery rate was 102.02% and the RSD was 3.18%.CONCLUSION The method is sensitive,specific and reproducible,which can be used to determine the content of calycosin-7-glucoside in Qifengtongqiao mixture.
作者
邓桂珠
卢泳
甘国兴
龙国斌
向燕芬
朱卫星
DENG Guizhu;LU Yong;GAN Guoxing;LONG Guobin;XIANG Yanfen;ZHU Weixing(Qingyuan Hospital of Traditional Chinese Medicine,Qingyuan,Guangdong 511500,China;Engineering Technology Research Center of Fresh Medicine and National Medicine of Guangdong Province,Qingyuan,Guangdong 511500,China)
出处
《今日药学》
CAS
2023年第9期690-693,共4页
Pharmacy Today
基金
2022年度清远市科技计划自筹经费项目(34)。
