摘要
益生元在促进益生菌生长、调节肠道微生态平衡方面发挥着重要作用。为探究兰州百合粗低聚糖(crude lily oligosaccharides,CLOs)的益生元特性,以兰州百合干为原料,提取、表征CLOs;以低聚果糖和菊粉作为阳性对照,测定CLOs在人工胃肠溶液的水解度,以及对嗜热链球菌ATCC-14485和保加利亚乳杆菌ATCC-11842的产酸、增殖作用的影响。结果表明:CLOs分子量为1194 Da,主要由甘露糖、阿拉伯糖和葡萄糖组成,摩尔比为1:3.57:16.40;CLOs经人工胃肠溶液消化后水解度是26.96%,显著低于菊粉(P<0.05);CLOs促进乳酸菌发酵产酸的效果显著优于菊粉和低聚果糖(P<0.05);当CLOs的添加量为1.0%(W/V)时,能有效促进嗜热链球菌和保加利亚乳杆菌的增殖,并减缓二者在货架期的死亡速率。研究结果说明CLOs具有益生元特性,可为新型共生发酵乳的研发提供理论依据。
Prebiotics play an important role in promoting the growth of probiotics and regulating the intestinal microflora balance.To explore the prebiotic characteristics of crude Lanzhou Lily(Lilium davidii var.unicolor)oli-gosaccharides(CLOs),the CLOs were extracted and characterized from dried Lanzhou lily,and the hydrolysis of CLOs in artificial gastrointestinal solution as well as the effects on acid production and proliferation of Streptococcus thermophilus ATCC-14485 and Lactobacillu bulgaricus ATCC-11842 were determined by using FOS and inulin as positive controls.The results showed:the molecular weight of CLOs was 1194 Da,and mainly consisted of man-nose,arabinose and glucose with a molar ratio of 1:3.57:16.40;the hydrolysis ratio of CLOs in artificial gastroin-testinal solution was 26.96%,which was significantly lower than that of inulin(P<0.05);the promotion effects of CLOs on acid production by lactic acid bacteria was significantly better than that of inulin and FOS(P<0.05);the addition of CLOs at 1.0%(W/V)could effectively promote the proliferation of S.thermophilus and L.bulgaricus,and slow down the death rate of both during the shelf life.These findings suggested that CLOs possess prebiotic charac-teristics,and can provide a theoretical basis for developing a novel symbiotic fermented milk.
作者
刘迪茹
唐绍琰
董尧
符瀛
马浩民
高琦宽
LIU Diru;TANG Shaoyan;DONG Yao;FU Ying;MA Haomin;GAO Qikuan(Institute of Nutrition and Food Hygiene,School of Public Health,Lanzhou University,Lanzhou 730000;Gansu Province Key Laboratory of Evidence Science Techniques Research and Application,Gansu University of Political Science and Law,Lanzhou 730070)
出处
《安徽农业大学学报》
CAS
CSCD
2023年第5期904-910,共7页
Journal of Anhui Agricultural University
基金
甘肃省科技计划项目(No.22JR5RA465)资助。
关键词
兰州百合粗低聚糖
益生元特性
嗜热链球菌
保加利亚乳杆菌
产酸
增殖
Lanzhou lily crude oligosaccharides
prebiotic properties
Streptococcus thermophilus
Lactoba-cillus bulgaricus
acid production
proliferation
