摘要
【目的】筛选高原鼢鼠(Eospalax baileyi)睾丸雌激素受体(Estrogen receptor,ER)免疫组化染色的适宜抗体浓度。【方法】研究选取10只成年雄性高原鼢鼠的睾丸,采用免疫组织化学SP法(IHC-SP法)检测雌激素受体α(ERα)和雌激素受体β(ERβ)在1∶150、1∶200和1∶250稀释浓度下的染色效果。【结果】ERα抗体稀释浓度1∶250的免疫组化平均光密度值显著高于1∶150和1∶200的抗体稀释浓度免疫组化平均光密度值(P<0.05),而ERβ抗体稀释浓度1∶200的平均光密度值最高(P<0.01),最适浓度结果均表现出阳性细胞染色强度高、背景清晰。【结论】高原鼢鼠睾丸中ERα和ERβ抗体稀释浓度分别为1∶250与1∶200的免疫组化染色效果最佳。
【Objective】This research aimed to determine the appropriate antibody concentration for immunohisto⁃chemical staining of estrogen receptor(ER)in testes of plateau zokor(Eospalax baileyi).【Method】We selected the testes of 10 adult male plateau zokors were selected to detect the staining effect of estrogen receptorα(ERα)and es⁃trogen receptorβ(ERβ)at three different dilution concentrations:1∶150,1∶200,and 1∶250 using the immunohisto⁃chemical SP method(IHC SP method).【Result】The mean immunohistochemical optical density of ERαantibody diluted at 1∶250 was significantly higher than that of antibody diluted at 1∶150 and 1∶200(P<0.05).In contrast,the immunohistochemical mean optical density of ERβantibody diluted at 1∶200 was the highest(P<0.01).At the opti⁃mal dilution concentration,positive cells exhibited intense staining with a clear background.【Conclusion】For the tes⁃tes of plateau zokors,the ideal antibody concentrations for ERαand ERβwere found to be 1∶250 and 1∶200,respec⁃tively,as these concentrations yielded the best immunohistochemical staining effects.
作者
包明芳
安康
谭宇尘
姚宝辉
康宇坤
王艳莉
张彩军
苏军虎
BAO Ming-fang;AN Kang;TAN Yu-chen;WANG Yan-li;YAO Bao-hui;KANG Yu-kun;ZHANG Cai-jun;SU Jun-hu(College of Grassland Science,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem,Ministry of Education,Grassland Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing Land Ecosystem Sustainability,Cansu Agricultural University-Massey University Research Centre for Grassland Biodiversity,Lanzhou 730070,China)
出处
《草原与草坪》
CAS
CSCD
2023年第5期99-103,共5页
Grassland and Turf
基金
国家自然科学基金(31460566,31760706)
陇原青年创新创业人才项目(LYRC2019-05)。
关键词
免疫组织化学SP法
抗体浓度
雌激素受体
immunohistochemical technique(IHC⁃SP method)
antibody concentration
estrogen receptor
