摘要
【目的】稻米直链淀粉含量(amylose content,AC)是决定稻米食味品质最为重要的因素之一。筛选具有低直链淀粉含量的水稻种质资源并探究其遗传机制,对于选育具有优质食味品质的水稻新品种具有重要意义。【方法】测定了22个三系杂交稻的恢复系品种的直链淀粉含量,并利用一个来源于恢复系繁38和申恢26杂交后代的双单倍体(Doubledhaploid,DH)群体,分析了控制直链淀粉含量的QTL位点。【结果】在22个恢复系中发现了2个低直链淀粉含量的品系繁32和繁38,其直链淀粉含量分别为11.39%±0.01%和10.58%±0.13%。与其他携带Wxmp等位基因的低直链淀粉材料不同,繁32和繁38携带Wxb等位基因。利用DH群体进行QTL分析,结果共检测到了5个控制直链淀粉含量的QTL位点(qAC1.1、qAC1.2、qAC5、qAC6.1和qAC6.2)。其中qAC6.1和qAC6.2两年均能检测到。qAC6.2是一个主效QTL位点,其表型贡献率超过30%。【结论】这些结果表明,繁38中低直链淀粉含量的表型可能主要由qAC6.2位点控制,可为深入研究水稻直链淀粉合成调节机理,及利用分子标记辅助选择技术选育低直链淀粉含量的水稻新品种奠定了基础。
【Objective】Amylose content(AC)is one of the most critical factors that determine the eating and cooking quality(ECQ)of rice.Therefore,it holds great significance to screen rice germplasm resources with low amylose content and elucidate the genetic control mechanism for breeding new varieties with excellent ECQ.【Method】We determined AC in 22 restorer lines of three-line hybrid rice and analyzed the QTL locus controlling amylose content using a double haploid(DH)population derived from the cross between Fan 38 and Shenhui 26.【Result】Two rice germplasms with low AC,Fan 32 and Fan 38,were discovered among the 22 restorer lines.Their AC was found to be 11.39%±0.01%and 10.58%±0.13%,respectively.In contrast to other low AC varieties carrying Wxmp alleles,Fan 32 and Fan 38 carried Wxb alleles.A total of five QTLs,namely qAC1.1,qAC1.2,qAC5,qAC6.1,and qAC6.2,related to amylose content,were identified in the DH population.Among these,qAC6.1 and qAC6.2 were consistently detectable over two years.qAC6.2 emerged as a major QTL,contributing to over 30%of the phenotypic variation.【Conclusion】These findings suggest that the low AC in Fan 38 is likely primarily controlled by the qAC6.2 locus.This establishes a foundation for further research into the regulatory mechanism of amylose synthesis in rice and for the development of new rice varieties with low AC using marker-assisted selection technology.
作者
谢开珍
张建明
程灿
周继华
牛付安
孙滨
张安鹏
闻伟军
代雨婷
胡启琰
邱越
曹黎明
储黄伟
XIE Kaizhen;ZHANG Jianming;CHENG Can;ZHOU Jihua;NIU Fuan;SUN Bin;ZHANG Anpeng;WEN Weijun;DAI Yuting;HU Qiyan;QIU Yue;CAO Liming;CHU Huangwei(College of Life Science and Fishery,Shanghai Ocean University,Shanghai 201306,China;Institute of Crop Breeding and Cultivation,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;Agricultural Technology Service Center of Zhangyan Town,Jinshan District,Shanghai,Shanghai 201514,China;Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops(Co-constructed by Ministry and Province),Ministry of Agriculture and Rural Affairs,Shanghai 201403,China)
出处
《中国水稻科学》
CAS
CSCD
北大核心
2023年第6期609-616,共8页
Chinese Journal of Rice Science
基金
上海市科技创新行动计划资助项目(21N11900100)
上海市水稻产业技术体系建设项目(沪农科产字2022第3号)。
