摘要
【目的】研究柽柳丛枝病在新疆南疆地区的分布危害、分类、形态特征及病原物16S rRNA基因的遗传多样性,为该病害的检测和鉴定提供理论依据。【方法】调查新疆南疆地区柽柳丛枝病的分布及危害,采用形态学与分子生物学相结合的方法,对柽柳丛枝病病原物进行透射电镜观察、16S rRNA基因和rp基因扩增,研究其形态特征及分类地位,并对新疆南疆不同地区柽柳丛枝病病原物16S rRNA基因遗传多样性。【结果】柽柳丛枝病在新疆南疆地区的平均发病率为9.06%,平均病情指数为5.66;在柽柳丛枝韧皮部组织中观察到有植原体颗粒的存在;16S rRNA基因和rp基因巢式PCR分别获得1219 bp和1174 bp大小的条带,柽柳丛枝病属于植原体病害,在分类地位上属于植原体16SrXXX-A亚组;新疆南疆柽柳丛枝植原体主要可分为4个株系,16S rRNA基因核苷酸相似度在96.5%~99.8%。【结论】柽柳丛枝病在新疆南疆地区主要分布在阿克苏市、温宿县、巴楚县、图木舒克市、阿拉尔市、伽师县,阿拉尔市12团等地,且发生情况和危害程度均较轻,不同地区之间的发病率及病情指数存在显著差异性;柽柳丛枝病存在植原体颗粒并主要在韧皮部组织中,有球形、椭圆形和不规则形态,大小300~600 nm不等。
【Objective】To clarify the distribution,taxonomic status,morphological characteristics and genetic differentiation of 16S rRNA of salt cedar witches'-broom disease in southern Xinjiang.【Methods】In this study,the distribution and damage of salt cedar witches'-broom(SCWB)disease in southern Xinjiang were investigated and the pathogens of SCWB was observed by transmission electron microscope,16S rRNA gene and rp gene amplification.Meanwhile,the molecular identification of the gene was carried out to clarify its morphological characteristics and taxonomic status,and the genetic differentiation of the 16S rRNA gene of SCWB in different areas of southern Xinjiang was studied.【Results】The average incidence of SCWB in southern Xinjiang was 9.06%,and the average disease index was 5.66.The existence of phytoplasma granules was observed in the phloem tissue of tamarisk branches.Nested PCR of 16S rRNA and rp gene bands with sizes of 1,219 bp and 1,174 bp were obtained respectively.Through homology comparison and phylogenetic tree construction,it was confirmed that the SCWB belonged to the phytoplasma disease,and belonged to the phytoplasma 16SrXXX-A subgroup in terms of taxonomic status;SCWB phytoplasma could be mainly divided into four lines,and the nucleotide similarity of 16S rRNA gene was between 96.5%and 99.8%in southern Xinjiang.【Conclusion】In this study,a systematic study of the phytoplasma disease of SCWB is carried out,the taxonomic status of the pathogen,the distribution and damage of the disease in southern Xinjiang are clarified,and the genetic differentiation of its 16S rRNA gene is analyzed.The rp gene i amplified to increase the content of phytoplasma diseases in the 16SrXXX group,which has provided a theoretical basis for the detection and identification of the disease.
作者
李丰
赖刚刚
赵志惠
陈小飞
朱天生
LI Feng;LAI Ganggang;ZHAO Zhihui;CHEN Xiaofei;ZHU Tiansheng(Key Laboratory of Integrated Pest Management(IPM)of Xinjiang Production and Construction Corps in Southern Xinjiang/Scientific Observing and Experimental Station of Crop Pests in Alar,Ministry of Agriculture,China/College of Agronomy,Tarim University,Aral Xinjiang 843300,China;Institute of Agriculture Sciences,the Fourth Agriculture Production Division of Xinjiang Production and Construction Crops,Cocodala Xinjiang 835219,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2023年第10期2551-2557,共7页
Xinjiang Agricultural Sciences
基金
国家自然科学基金项目(31060238)
新疆生产建设兵团特色果树生产工程实验室开放课题(FE202001)
新疆生产建设兵团财政科技计划项目(2019DA001,2020DA003)。
