摘要
为探讨鸭昼夜节律相关基因RORB(视黄醇相关孤儿受体B)与鸭蛋重性状的相关性,采用Illumina测序的方法对90只金定鸭RORB基因序列进行测序,以分析RORB基因上3个SNP位点在不同蛋重鸭个体的差异,并与300日龄和450日龄蛋重进行相关性分析;荧光定量PCR的方法检测RORB及相关基因CLOCK、BMAL1和PER2 mRNA在不同蛋重鸭卵巢组中的表达差异。结果表明,RORB基因上Z-35251072和Z-35256947位点的SNP突变与鸭450日龄的蛋重呈显著负相关,Z-35278196位点的SNP突变与鸭300日龄和450日龄蛋重呈显著负相关;RORB和PER2在低蛋重组中的表达显著高于在中蛋重和高蛋重组的表达(P<0.05),而CLOCK和BMAL1在高蛋重组中的表达显著高于中蛋重和低蛋重组的表达(P<0.05)。结果提示,RORB在鸭的蛋重性能中可能发挥重要作用,且可能与昼夜节律相关基因间的相互调节有关,该结果为研究鸭的蛋重性能调控奠定理论基础。
The aim of this study was to explore the correlation between duck circadian rhythm related gene RORB(retinoid related orphan receptor B)and duck egg weight.Illumina sequencing was used to sequence the RORB gene of 90 ducks to analyze the differences in three SNP loci on the RORB gene among ducks with different egg weights,and to analyze the correlation between the RORB gene and the weight of 300 day and 450 day eggs.The expression of RORB and related genes CLOCK,BMAL1 and PER2 mRNA in the ovaries of the ducks with different egg weight was detected by fluorescence quantitative PCR.The results showed that the SNP mutations at the Z-35251072 and Z-35256947 of RORB genes were significantly negatively correlated with the 450 day egg weight of ducks.The SNP mutations at Z-35278196 of RORB gene were negatively correlated with the egg weight of ducks at 300 d and 450 d.The expression of RORB and PER2 in the low egg weight group was significantly higher than that in the middle and high egg weight groups(P<0.05),while the expression of clock and BMAL1 in the high egg weight group was significantly higher than that in the middle and low egg weight groups(P<0.05).These results suggested that RORB might play an important role in duck egg production performance,and might be related to the mutual regulation of circadian rhythm related genes,which laid a theoretical foundation for regulation of duck egg weight.
作者
陶志云
朱春红
徐文娟
章双杰
宋卫涛
刘宏祥
屠琦
王志成
李慧芳
TAO Zhiyun;ZHU Chunhong;XU Wenjuan;ZHANG Shuangjie;SONG Weitao;LIU Hongxiang;TU Qi;WANG Zhicheng;LI Huifang(Jiangsu Institute of Poultry Science,Yangzhou 225125,China;Suzhou Customs Comprehensive Technology Center,Suzhou 215100,China)
出处
《畜牧与兽医》
CAS
北大核心
2023年第11期11-15,共5页
Animal Husbandry & Veterinary Medicine
基金
江苏省种业振兴“揭榜挂帅”项目(JBGS[2011]111)
江苏省现代农业(水禽)产业技术体系项目(JATS[2022]404)
国家家养动物种质资源库项目(2023)。
