摘要
目的:利用非靶向代谢组学方法,分析口源性口臭患者和健康人舌苔代谢物差异,寻找口源性口臭差异显著代谢物作为生物标志物。方法:采用液相色谱质谱技术结合主成分分析法和正交偏最小二乘法-判别分析法,分别对12例口源性口臭患者和12名健康人舌苔样本进行非靶向代谢组学研究,以正交偏最小二乘法-判别分析法模型中变量投影重要性值>1和t检验P<0.05为标准筛选并确定差异性代谢物。结果:口源性口臭患者和健康人舌苔代谢物存在差异,鉴定到11个差异代谢物,谷氨酸、甘氨酸-苯丙氨酸、色氨酰-脯氨酸、脱氧腺苷、4,5-二氢白色向日葵素、N-乙酰-DL-色氨酸、帕拉米松乙酸酯、环戊醇、(2-己基环戊亚基)肼硫代甲酰胺、L-哌可酸和牛磺酸。其中口源性口臭组中的甘氨酸-苯丙氨酸和N-乙酰-DL-色氨酸表达显著上调,而牛磺酸表达显著下调。结论:口源性口臭患者和健康人的舌苔代谢物存在差异,具有诊断价值的差异代谢物或可作为口源性口臭的诊断标志物。
PURPOSE:To analyze the difference of metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals by untargeted metabolomics,and to explore significant differences in metabolites of intra-oral halitosis as biomarkers.METHODS:The untargeted metabolomics of tongue coating samples from 12 patients with intra-oral halitosis and 12 healthy individuals were studied by liquid chromatography and mass spectrometry combined with principal component analysis and orthogonal partial least squares discriminant analysis.The value of variable importance in projection>1 and P<0.05 of Student's t test in the orthogonal partial least squares-discriminant analysis model were used as the criteria to screen and determine the differential metabolites.RESULTS:There were differences in the metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals,and 11 different metabolites were identified.They were valyl-arginine,glycine-phenylalanine,tryptophyl-proline,deoxyadenosine,4,5-dihydroniveusin A,N-acetyl-DL-tryptophan,paramethasone acetate,cyclopentanol,[(2-hexylcyclopentylidene)amino]thiourea,L-pipecolic acid and taurine.In the intra-oral halitosis group,the expressions of Glycine-phenylalanine and N-acetyl-DL-tryptophan were significantly up-regulated,while the expressions of taurine were significantly down-regulated.CONCLUSIONS:There are differences in the metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals.The differential metabolites with diagnostic value may be used as diagnostic markers of intra-oral halitosis.
作者
Aviella Nathania Liman
朱宏来
杨雯洁
叶玮
LIMAN Aviella-nathania;ZHU Hong-lai;YANG Wen-jie;YE Wei(Department of Preventive Dentistry,Shanghai Ninth People's Hospital,Shanghai Jiaotong University School of Medicine,College of Stomatology,Shanghai Jiaotong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology.Shanghai 200011;Xinzhuang Community Health Service Center,Minhang District.Shanghai 201199,China)
出处
《上海口腔医学》
CAS
北大核心
2023年第5期525-531,共7页
Shanghai Journal of Stomatology
关键词
口源性口臭
舌苔
代谢组学
生物标志物
Intra-oral Halitosis
Tongue coating
Metabolomics
Biomarker
