摘要
目的探究系统性红斑狼疮(SLE)患者血清肿瘤坏死因子受体相关因子3(TRAF3)、微小RNA(miR)-107表达水平及与疾病活动度的关系。方法纳入该院2020年1月至2022年1月收治的80例SLE患者为SLE组。根据SLE疾病活动指数(SLEDAI)评分进一步将研究对象分为轻中度组32例(SLEDAI评分<10分)和重度组48例(SLEDAI评分≥10分)。另纳入同期体检健康者80例为对照组。采用荧光定量PCR测定血清TRAF3、miR-107的表达水平,采用Pearson法分析血清中TRAF3、miR-107表达水平与实验室指标的相关性,采用受试者工作特征(ROC)曲线评价血清TRAF3、miR-107联合对重度SLE患者发生的预测效能。结果SLE组血清中TRAF3表达水平显著高于对照组,miR-107表达水平显著低于对照组,差异有统计学意义(P<0.05)。相较于轻中度组,重度组发热脱发比例、SLEDAI评分、白细胞介素(IL)-6、IL-1β、肿瘤坏死因子-α(TNF-α)、C反应蛋白(CRP)、TRAF3显著较高,miR-107、血小板(PLT)、补体C3(C3)、C4水平显著较低,差异有统计学意义(P<0.05)。SLE患者血清TRAF3与SLEDAI评分、IL-6、IL-1β、TNF-α呈正相关(r=0.680、0.537、0.556、0.574,P<0.05),与PLT呈负相关(r=-0.527,P<0.05),与C3、C4、CRP呈弱相关(r=-0.380、-0.337、0.245,P<0.05)。血清miR-107与TRAF3、SLEDAI评分、IL-6、IL-1β、TNF-α呈负相关(r=-0.581、-0.692、-0.541、-0.563、-0.579,P<0.05),与PLT呈正相关(r=0.536,P<0.05),与C3、C4、CRP呈弱相关(r=0.392、0.351、-0.273,P<0.05)。ROC结果显示,血清TRAF3、miR-107表达水平及二者联合预测发生重度SLE的AUC分别为0.760、0.762、0.850,其中联合预测AUC显著高于二者单独预测的AUC,差异有统计学意义(Z=1.269、1.201,P<0.05)。结论SLE患者血清TRAF3表达水平较高、miR-107表达水平较低,均与患者SLEDAI评分、PLT、IL-6、IL-1β、TNF-α有关,能够反映SLE疾病活动度,有望成为重度SLE发生的预测因子。
Objective To investigate the expression of tumor necrosis factor receptor-associated factor 3(TRAF3)and microRNA-107 in patients with systemic lupus erythematosus(SLE)and their relationship with disease activity.Methods The 80 SLE patients admitted to Affiliated Hospital of Jiangnan University from January 2020 to January 2022 were included in the SLE group.According to the SLE disease activity index(SLEDAI)score,the subjects were further divided into mild to moderate group of 32 cases(SLEDAI<10)and severe group of 48 cases(SLEDAI≥10).In addition,80 patients with physical examination were included as the control group.The expression levels of serum TRAF3 and miR-107 were measured by fluorescence quantitative PCR.Pearson method was applied to analyze the correlation between the expression levels of TRAF3 and miR-107 in serum and laboratory indicators.ROC curve was applied to evaluate the predictive efficacy of the combination of TRAF3 and miR-107 in serum for the occurrence of severe SLE.Results The serum levels of TRAF3 and miR-107 in SLE group were obviously higher than those in control group,and the differences were statistically significant(P<0.05).Compared with mild and moderate group,the proportion of fever and alopecia,SLEDAI score,interleukins(IL-6),IL-1β,tumor necrosis factor(TNF-α),C-reactive Protein(CRP)and TRAF3 in severe group were obviously higher,and the levels of miR-107,platelet(PLT),complement 3(C3)and C4 were obviously lower,and the differences were statistically significant(P<0.05).Serum TRAF3 was positively correlated with SLEDAI score,IL-6,IL-1βand TNF-α(r=0.680,0.537,0.556,0.574,P<0.05),negatively correlated with PLT(r=-0.527,P<0.05),and weakly correlated with C3,C4 and CRP(r=-0.380,-0.337,0.245,P<0.05).Serum miR-107 was negatively correlated with TRAF3,SLEDAI score,IL-6,IL-1β,TNF-α(r=-0.581,-0.692,-0.541,-0.563,-0.579,P<0.05),positively correlated with PLT(r=0.536,P<0.05),and weakly correlated with C3,C4,CRP(r=0.392,0.351,-0.273,P<0.05).The ROC analysis results showed that the AUC o
作者
汤茜
宋冬明
王方明
刘敏黎
TANG Xi;SONG Dongming;WANG Fangming;LIU Minli(Department of Rheumatology and Immunology,Affiliated Hospital of Jiangnan University,Wuxi,Jiangsu 214000,China)
出处
《国际检验医学杂志》
CAS
2023年第21期2607-2611,共5页
International Journal of Laboratory Medicine
基金
江南大学附属医院临床研究与转化医学研究项目(LCYJ202213)。
