摘要
目的 分析循环肿瘤细胞(CTC)与三阴性乳腺癌(TNBC)预后及肿瘤状态的关系。方法 从32例Ⅲ~Ⅳ期TNBC患者和30名健康对照者的外周血中分离出CTC,进行分子特征分析。结果 与3.2 mL血样中CTC计数<5个的23例患者相比,CTC计数≥5个的8例患者无进展生存期、总生存期均更差,差异有统计学意义(P=0.033、0.006),表明CTC计数对TNBC患者预后具有评估价值;3.2 mL血样中CTC计数≥5个的患者中,CTC簇的存在与患者较差的总生存期有关(P=0.001)。CTC的上皮、间充质和干性标志物基因表达显示出重要的细胞可塑性,表明上皮间充质转化的中间表型与肿瘤的有效转移有关,且CTC的分子特征可以有效预测患者预后。结论 CTC的分子特征能够反映晚期TNBC患者的肿瘤状态,或可作为高度特异的生物标志物用于无创预后监测。
Objective To analyze the relationships of circulating tumor cell(CTC)with prognosis and tumor status of triple-negative breast cancer(TNBC).Methods CTC was isolated from the peripheral blood of 32 patients confirmed as TNBC and 30 healthy controls,and molecular characteristics were analyzed.Results Compared with 23 patients with CTC count<5 in 3.2 mL blood samples,8 patients with CTC count≥5 had worse progression-free survival and overall survival,and the difference was statistically significant(P=0.033,0.006),suggesting that the CTC count was valuable in evaluating the prognosis of TNBC patients;among patients with CTC count≥5 in 3.2 mL blood samples,the presence of CTC clusters was associated with poorer overall survival(P=0.001).Epithelial,mesenchymal,and dry marker gene expression of CTC showed important cellular plasticity,suggesting that the intermediate phenotype of epithelial mesenchymal transformation was associated with effective tumor metastasis,and the molecular characteristics of CTC can effectively predict patients′prognosis.Conclusion The molecular features of CTC can reveal the tumor status of patients with advanced TNBC and can be used as highly specific biomarkers with the potential for noninvasive prognostic monitoring.
作者
王浩
李南林
曹海玲
WANG Hao;LI Nanlin;CAO Hailing(Department of Pathology,Panyu District Central Hospital of Guangdong Province,Guangzhou,Guangdong,510000;Department of Thyroid Gland and Mammary Gland Surgery,Xijing Hospital of Air Force Military Medical University of Chinese People′s Liberation Army,Xi′an,Shaanxi,710032;Department of Thyroid Gland and Mammary Gland Surgery,Panyu District Central Hospital of Guangdong Province,Guangzhou,Guangdong,510000)
出处
《实用临床医药杂志》
2023年第18期39-43,共5页
Journal of Clinical Medicine in Practice
基金
国家自然科学基金青年项目(81602608)。
关键词
循环肿瘤细胞
三阴性乳腺癌
预后
转移
上皮间充质转化
circulation tumor cells
triple-negative breast tumor
prognosis
metastasis
epithelial mesenchymal transformation
