摘要
As important yield-related traits,thousand-grain weight(TGW),grain number per spike(GNS)and grain weight per spike(GWS)are crucial components of wheat production.To dissect their underlying genetic basis,a double haploid(DH)population comprised of 198 lines derived from 8762/Keyi 5214 was constructed.We then used genechip to genotype the DH population and integrated the yield-related traits TGW,GNS and GWS for QTL mapping.Finally,we obtained a total of 18942 polymorphic SNP markers and identified 41 crucial QTLs for these traits.Three stable QTLs for TGW were identified on chromosomes 2D(QTgw-2D.3 and QTgw-2D.4)and 6A(QTgw-6A.1),with additive alleles all from the parent 8762,explaining 4.81–18.67%of the phenotypic variations.Five stable QTLs for GNS on chromosomes 3D,5B,5D and 6A were identified.QGns-5D.1 was from parent 8762,while the other four QTLs were from parent Keyi 5214,explaining 5.89–7.08%of the GNS phenotypic variations.In addition,a stable GWS genetic locus QGws-4A.3 was detected from the parent 8762,which explained 6.08–6.14%of the phenotypic variations.To utilize the identified QTLs,we developed STARP markers for four important QTLs,Tgw2D.3-2,Tgw2D.4-1,Tgw6A.1 and Gns3D.1.Our results provide important basic resources and references for the identification and cloning of genes related to TGW,GNS and GWS in wheat.
基金
the Henan Modern Agricultural Industrial Technology System Construction,China(HARS-22-1-Z7)。
