摘要
融合魏斯氏菌(Weissella confusa)ZW21是1株具有良好抗菌活性的牦牛乳源乳酸菌,为探索其抗菌机理,分析其全基因组序列并挖掘该菌株的抗菌等功能特性基因。本研究基于Illumina NovaSeq PE150平台对W.confusa ZW21进行全基因组测序分析,并采用基因功能、京都基因与基因组百科全书、蛋白质直系同源簇、非冗余蛋白、碳水化合物活性酶数据库、转运蛋白分类数据库进行基因组基本功能注释。结果表明:W.confusa ZW21的基因组大小为2.44 Mb,GC含量45.66%;预测到2175个编码基因,编码基因总长度为1947771 bp,平均长度为896 bp;通过功能数据库对该菌株基因组基本功能注释和代谢通路基因信息注释可知,基因组中含有8种细菌素(如大肠菌素V和乳球菌素)相关基因、3种与抗氧化活性相关基因,以及可调控免疫和炎症信号通路(如核苷酸结合寡聚化结构域样受体蛋白受体信号通路、过氧化物酶体增殖物激活受体信号通路)和编码抗叶酸的基因。综上,W.confusa ZW21全基因组测序及分析为研究其抗菌机理提供基础。
In order to explore the antibacterial mechanism of Weissella confusa ZW21,a strain of lactic acid bacteria from yak milk with good antibacterial activity,its whole genome sequence was analyzed and its antibacterial activity-related functional genes were mined.In this study,the whole genome of W.confusa ZW21 was sequenced and analyzed on the Illumina NovaSeq PE150 platform,and the basic functions of the genome were annotated in the Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG),Cluster of Orthologous Groups,Non-Redundant Protein databases.The results showed that:(1)the genome size of W.confusa ZW21 was 2.44 Mb,and the GC content was 45.66%;(2)2175 encoding genes were predicted,with a total length of 1947771 bp and an average length of 896 bp;and(3)the encoding genes were annotated in the functional database for basic functional annotation and metabolic pathway gene information annotation of the strain’s genome,revealing that the genome contained eight bacteriocin-related genes(such as Colicin V and Lactococcin);three antioxidant activity-related genes;signaling pathways that regulate immunity and inflammation such as the nucleotide oligomerization domain(NOD)-like receptor and peroxisome proliferators-activated receptor(PPAR)signaling pathways,and antifolate encoding genes.In summary,the whole genome sequencing and analysis of W.confusa ZW21 can provide a basis for studying its antibacterial mechanism.
作者
郑雪
梁琪
宋雪梅
张炎
ZHENG Xue;LIANG Qi;SONG Xuemei;ZHANG Yan(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China;Functional Dairy Product Engineering Laboratory of Gansu,Lanzhou 730070,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第20期119-126,共8页
Food Science
基金
国家自然科学基金地区科学基金项目(31660468)。
关键词
牦牛乳
融合魏斯氏菌
全基因组测序
基因功能注释
细菌素
yak milk
Weissella confusa
whole genome sequencing
gene function annotation
bacteriocin
