摘要
目的探讨节律基因昼夜节律运动输出周期故障(CLOCK)单核苷酸多态性与生活方式对学龄前儿童肥胖的作用。方法选择2021年6月至2022年6月在佛山复星禅诚医院儿童保健门诊查体的肥胖儿童60例(肥胖组),其中男性34例,女性26例;年龄3~6岁,平均年龄5.24岁;体质量16.54~18.73 kg,平均体质量17.08 kg。另选择100例体质量正常的健康儿童作为对照组,其中男性57例,女性43例;年龄3~6岁,平均年龄5.39岁;体质量13.75~15.21 kg,平均体质量14.96 kg。检测两组儿童节律基因CLOCK单核苷酸T3111C位点多态性基因型及等位基因频率分布,通过调查问卷统计两组儿童的临床资料和生活方式。采用多因素Logistic回归模型分析肥胖的危险因素,估算CLOCK单核苷酸T3111C位点多态性基因型和生活方式与肥胖风险的调整比值比(OR)及95%可信区间(CI),分析节律基因CLOCK单核苷酸T3111C位点多态性与生活方式因素对学龄前儿童肥胖的交互作用。结果两组儿童TT基因型频率、TC基因型频率、CC基因型频率差异均有统计学意义(肥胖组与对照组比,86.67%vs 63.00%,10.00%vs 25.00%,3.33%vs 13.00%。P<0.05)。两组儿童基因T分布和基因C分布(91.67%vs 75.00%,8.33%vs 25.00%),差异均有显著统计学意义(P<0.001)。两组在出生体质量、暴饮暴食、屏幕接触时间、户外活动时间、豆制品、海产品、垃圾食品方面差异有统计学意义(P<0.05)。暴饮暴食、户外活动时间<30 min/d、垃圾食品≥3次/周、豆制品<3次/周、节律基因CLOCK单核苷酸T3111C位点携带TT基因型是影响学龄前儿童肥胖的独立危险因素。节律基因CLOCK单核苷酸T 3111C位点TT基因型与暴饮暴食、户外活动时间、豆制品及垃圾食品摄入量均有正交互作用。结论暴饮暴食、户外活动时间<30 min/d、垃圾食品≥3次/周、豆制品<3次/周、节律基因CLOCK单核苷酸T3111C位点携带TT基因型是影响学龄前儿童肥胖的独立危
Objective To explore the interaction between circadian gene CLOCK single nucleotide polymorphism and lifestyle in preschool obesity.Methods From June 2021 to June 2022,60 obese children(obese group)examined in Child Health Clinic were enrolled,which included 34 males and 26 females,aged 3-6 years old with mean age of 5.42 years old;body mass was 16.54-18.73 kg with mean body mass of 17.08 kg.Another 100 healthy children with normal body mass were set as control group,which included 57 males and 43 females,aged 3-6 years old with mean age of 5.39 years old;body mass was 13.75-15.21 kg with mean body mass of 14.96 kg.The genotype and allele frequency distribution of CLOCK single nucleotide polymorphism T3111C were detected in 2 groups,the general data and lifestyle of 2 groups were counted by questionnaire.The multivariate Logistic regression model was used to analyze risk factors of obesity,the adjusted odds ratio(OR)and 95%confidence interval(CI)of CLOCK single nucleotide polymorphism T3111C genotype and lifestyle with obesity risk were estimated,the interaction between CLOCK single nucleotide polymorphism T3111C and lifestyle factors on obesity in preschool children was analyzed.Results There were significant differences in TT genotype frequency,TC genotype frequency and CC genotype frequency between obese group and control group(86.67%vs 63.00%,10.00%vs 25.00%,3.33%vs 13.00%.P<0.05).There were significant differences in gene T distribution and gene C distribution between 2 groups(91.67%vs 75.00%,8.33%vs 25.00%.P<0.001).There were significant differences in birth body mass,overeating,screen contact time,outdoor activity time,bean products,seafood and junk food between 2 groups(P<0.05).The overeating,outdoor activity time<30 minutes/day,junk food≥3 times/week,bean products<3 times/week,T3111C carrying TT genotype were independent risk factors for obesity in preschool children.The TT genotype of CLOCK single nucleotide T3111C locus showed positive interaction with overeating,outdoor activity time,bean products a
作者
刘玲
林秀雯
LIU Ling;LIN Xiu-wen(Department of Child Health Care,Foshan Fosun Chancheng Hospital,Foshan 528000,Guangdong,China)
出处
《生物医学工程与临床》
CAS
2023年第5期656-662,共7页
Biomedical Engineering and Clinical Medicine
基金
佛山市卫生健康局医学科研课题(20220809A010033)。
关键词
单核苷酸多态性
节律基因
生活方式
儿童肥胖
交互作用
single nucleotide polymorphism
circadian gene
life style
childhood obesity
interaction
