摘要
为建立杉木无性系发育技术体系,以广西杉木为试验材料,选择树势基本相同、生长健壮、无病虫害的杉木为外植体进行组培快繁技术试验研究。结果表明:(1)外植体最佳消毒方案是使用70%酒精浸泡15 s,灭菌后采用无菌水洗涤3次,再用0.1%升汞浸泡消毒8 min后采用无菌水洗涤5次;(2)初代诱导最佳培养基为1/2MS+6-BA0.6 mg·L^(-1),该培养基有利于增加培养基的诱导率和萌芽率,提高培养基的培养效果;(3)增殖诱导最适培养基为1/2MS+6-BA0.6 mg·L^(-1)+IBA0.3 mg·L^(-1),获得的组培苗芽多,茎粗壮;(4)生根最适培养基为1/2MS+IBA0.8 mg·L^(-1)+IAA0.1 mg·L^(-1);(5)杉木组培苗移栽的最适基质是泥炭土∶菜园土∶蛭石=4∶2∶1,其幼苗成活率达到91.93%,幼苗健壮,叶色深绿。
In order to establish a technical system for the development of Guangxi fir clones,the experiment took Guangxi fir as the research material,and selected Guangxi fir with basically the same vigor,robust growth,and no pests and diseases as explants to carry out tissue culture rapid propagation technology research.The results showed as the following five aspects.(1)The optimal disinfection scheme of explants was to soak in 70%alcohol for fifteen seconds,wash with sterile water for three times after sterilization,soak and disinfect with 0.1%mercuric chloride for eight minutes,and then wash with sterile water for five times.(2)The best medium for primary induction was 1/2MS+6-BA0.6mg·L^(-1),which was beneficial to increasing the induction rate and germination rate of the medium and improving the culture effect of the medium.(3)The optimal medium for proliferation induction was 1/2MS+6-BA0.6mg·L^(-1)+IBA0.3mg·L^(-1),the obtained tissue culture seedlings had many buds and thick stems.(4)The optimal medium for rooting was 1/2MS+IBA0.8mg·L^(-1)+IAA0.1mg·L^(-1).(5)The most suitable substrate for transplanting Guangxi fir tissue culture seedlings was peat soil:vegetable garden soil:vermiculite=4:2:1,by which survival rate of seedlings reached 91.93%.Guangxi fir seedlings were robust and the leaves were dark green.
作者
郭秀丽
Guo Xiuli(Guangxi Zhuang Autonomous Region State-owned Huangmian Forest Farm,Liuzhou 545600,China)
出处
《防护林科技》
2023年第6期45-49,共5页
Protection Forest Science and Technology
关键词
广西杉木
组织培养
培养基
增殖培养
Guangxi fir
tissue culture
culture medium
proliferation culture
