摘要
日益提升的环境生态和人体健康理念对市政污水处理厂综合毒性减排提出了挑战,其中特征毒害污染物的识别是出水安全性评估的难点.腺苷三磷酸结合盒转运蛋白(ATP-binding cassette transporter,ABC转运蛋白)是生物细胞中有毒物质的外排泵,在细胞外源性防御系统中扮演关键角色,但关于污水中ABC转运蛋白抑制剂类污染物的认知仍十分有限.本研究应用ABC转运蛋白活性导向的效应导向分析方法,对长江流域33个采用不同污水处理主体工艺的市政污水厂的进出水开展了毒性评价和关键致毒物质筛查研究.结果表明,大部分市政进水具有转运蛋白抑制效应,但绝大多数市政出水不具有抑制效应.对于工艺脱毒能力,厌氧-缺氧-好氧(A^(2)O)、改良氧化沟和改良序批式反应器(SBR)稳定削减了大部分ABC转运蛋白抑制效应,多级串联A^(2)O及其与膜生物反应器(MBR)的组合将整体工艺脱毒率提升至100%,但厌氧-好氧工艺(AO)和倒置A^(2)O的工艺脱毒率不佳.A^(2)O处理后主要生成低极性低毒小分子化合物,而倒置A^(2)O工艺处理后生成以中低极性化合物为主的有毒副产物.对武汉、南京和上海的代表性市政进出水的非靶向分析共识别到1431种可疑化合物,药物类相对丰度最高(42.3%~46.5%),其次是农药、工业化学品和个人护理品.化工医药有机原料或中间体、激素、心血管药物和杀虫剂是与市政污水ABC转运蛋白抑制削减密切相关的关键物质类别.本研究可为全面评估长江流域污水处理厂的出水水质提供基础毒理学信息,并为污水处理厂毒性减排工艺设计和新污染物风险管控提供理论指导.
The increasingly improved concept of environmental ecology and human health poses a challenge to the comprehensive toxicity reduction of municipal wastewater treatment plants(WWTPs),among which the identification of toxic pollutants is the difficulty in the assessment of effluent safety.The ATP-binding cassette(ABC)transporter is the efflux pump of toxic substances in the cell membrane and plays a key role in the cellular exogenous defense system.However,there is still a very limited understanding of the ABC transporter inhibitors in wastewater.This study applied effect-directed analysis based on ABC transporter activity to evaluate toxicity and screen key substances in influents and effluents of 33 municipal WWTPs with different treatment processes in the Yangtze River Basin.The results showed that most influents could inhibit the activity of the ABC transporter,while most effluents had no inhibitory effects.For detoxification capacities of the different processes,the anaerobic-anoxic-aerobic(A^(2)O),improved oxidation ditch,and improved sequencing batch reactor(SBR)reduced most of the inhibition effects on ABC transporters.The tandem multistage A^(2)O and its combination with membrane bioreactor(MBR)increased the overall detoxification rate to 100%.However,the detoxification capacities of the anaerobic aerobic process(AO)and inverted A^(2)O processes were not good.The small molecule compounds with low polarity and low toxicity were mainly generated after A^(2)O treatment,while the toxic by-products with low and medium polarity compounds are mainly generated after inverted A^(2)O treatment.Non-targeted analysis showed that a total of 1431 suspected compounds were identified from the representative influents and effluents of WWTPs in Wuhan,Nanjing,and Shanghai.The relative abundance of drugs was the highest(42.3%~46.5%),followed by pesticides,industrial chemicals,and personal care products.Chemical and pharmaceutical organic raw materials or intermediates,hormones,cardiovascular drugs,and pesticides were the key
作者
吴星悦
何若男
穆洪新
陈玲
吴兵
任洪强
WU Xingyue;HE Ruonan;MU Hongxin;CHEN Ling;WU Bing;REN Hongqiang(State Key Laboratory of Pollution Control and Resource Reuse,School of Environment,Nanjing University,Nanjing 210023)
出处
《环境科学学报》
CAS
CSCD
北大核心
2023年第10期15-25,共11页
Acta Scientiae Circumstantiae
基金
国家自然科学基金项目(No.22022604)
江苏省自然科学基金项目(No.BK20200011)
南京大学卓越计划项目(No.ZYJH005)。
关键词
市政污水
ABC转运蛋白
效应导向分析
非靶向筛查
municipal wastewater
ABC transporter
effect-directed analysis
non-targeted screening
