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致犊牛腹泻大肠杆菌的分离培养与药敏试验

Experimental Report on Prevention and Treatment of Stubborn Diarrhea in Calves
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摘要 为进一步探讨防止犊牛顽固性腹泻的防治方案,根据某养殖场的发病案例,采用细菌分离法分离病料中的大肠杆菌,明确大肠杆菌的致病性,并采用纸片扩散法进行了大肠杆菌的药敏试验,筛选敏感药物。结果表明,案例中共分离到75株致病性大肠杆菌;不同的大肠杆菌菌株对药物的敏感性存在一定差异;90%以上的菌株对头孢噻吩、环丙沙星、恩诺沙星、左氧氟沙星高敏,60%左右的菌株对氨苄西林、庆大霉素、阿米卡星、卡那霉素高敏,60%左右的菌株对复方新诺明和红霉素中敏,40%的菌株对四环素、链霉素耐药。因此,在控制疫病的过程中,必须对病因进行准确诊断,并进行严格的药敏实验,以确定敏感性的药品,再进行有针对性的治疗。 in order to further explore the prevention and treatment of intractable diarrhea in calves,according to a case of illness in a farm,Escherichia coli was isolated from the sick materials by the method of bacterial isolation,and the pathogenicity of E.coli was determined,the paper disc diffusion method was used to carry out the drug sensitivity test of E.coli and screen the sensitive drugs.The results showed that a total of 75pathogenic E.coli strains were isolated,and the drug sensitivity of different E.coli strains was different More than 90%of the isolates were sensitive to cefalotin,Ciprofloxacin,enrofloxacin and levofloxacin,while about 60%were sensitive to ampicillin,gentamicin,Amikacin and kanamycins Gao Min,about 60%were sensitive to Trimethoprim/sulfamethoxazole and erythromycin,and 40%were resistant to tetracycline and streptomycin.Therefore,in the process of controlling the epidemic disease,we must make an accurate diagnosis of the cause of the disease,and carry out strict drug sensitivity test to determine the sensitive drugs,and then carry out targeted treatment.
作者 思汗 哈日巴拉 郝剑峰 Sihan;Haribala;HAO Jianfeng(Hulunbuir Animal Disease Control Center,Hulunbuir 021008,China;Inner Mongolia Animal Disease Control Center,Hohhot 010020,China)
出处 《兽医导刊》 2023年第1期53-56,共4页 Veterinary Orientation
关键词 犊牛 顽固性腹泻 病原鉴定 防治方案 Calf Refractory diarrhea Pathogen identification Prevention and control plan
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