摘要
目的:基于仿生学原理设计、合成具备黏附性能的阴、阳离子肽,并分析其混合后对黏附力的影响,为新型仿生黏附材料研制奠定基础。方法:分析贻贝足蛋白5(mussel foot protein 5,Mfp5)和沙堡蠕虫分泌蛋白3(cement precursor protein of the Phragmatopoma californica 3,Pc3)的结构特点,理性设计出黏附性能较好的阴、阳离子肽。通过拉力强度试验机、原子力显微镜(atomic force microscope,AFM)及石英晶体微天平(quartz crystal microbalance,QCM)等对其黏附力和吸附量等参数进行检测,并从多肽组成和相互作用等方面分析其黏附机制。结果:阴、阳离子肽通过多肽固相合成法合成,纯度达到95%以上。拉力测试表明阴、阳离子肽的黏附力随固化时间增加而增大,最高分别达到174.04 kPa及180.11 kPa,并且两者混合后黏附力显著增强,等比例混合后高达347.81 kPa;QCM检测表明阳离子肽、阴阳离子肽混合物对金(aurum,Au)表面的吸附量可分别达到82.67 ng/cm^(2)和151.53 ng/cm^(2);AFM检测阴、阳离子肽的平均微观黏附力分别为5.43 nN及4.95 nN,且两者等比例混合后可提高至18.54 nN。结论:基于贻贝和沙堡蠕虫构建的阴、阳离子肽具备良好的黏附性能,且其黏附力可通过静电力介导的复合凝聚显著提高,该结果为新型仿生黏附材料研制提供了依据。
Objective:Inspired by the principle of bionics,both anionic and cationic peptides with adhesive properties are designed and analyzed the effect of their mixing on adhesion to lay the foundation for the development of new bionic adhesive materials.Methods:The structural characteristics of mussel foot protein 5(Mfp5)and cement precursor protein of the phragmatopoma californica 3(Pc3)were analyzed,according to which the anionic and cationic peptides with adhesion properties were designed and screened.The adhesive force and adsorption capacity of these peptides were tested by tensile strength testing machine,atomic force microscope(AFM)and Quartz crystal microbalance(QCM).Furthermore,their underlying adhesion mechanisms were analyzed in terms of their compositions and interactions.Results:The anionic and cationic peptides with the purity of over 95%were synthesized using the solid phase peptide synthesis method.The tensile test showed that the adhesive forces of anionic and cationic peptides increased with the extension of curing time,with the highest value reaching 174.04 kPa and 180.11 kPa,respectively.In addition,the adhesive force of the mixed anionic and cationic peptides was significantly enhanced compared to their own adhesive forces,and the adhesive force of their mixture mixed in equal proportions reached 347.81 kPa.The QCM results showed that the adsorption capacity of cationic peptide,and the mixture of both anionic and cationic peptides on gold(Au)surface reached 82.67 ng/cm^(2)and 151.53 ng/cm^(2),respectively.The average microscopic adhesions of anionic and cationic peptides detected by AFM were 5.43 nN and 4.95 nN,respectively,while the average microscopic adhesion of their mixture mixed in equal proportions reached 18.54 nN.Conclusion:The anionic and cationic peptides designed based on mussel and Phragmatopoma californica had certain adhesion properties,which could be significantly increased by electrostatic force mediated complex condensation.Therefore,our study provides a basis for the development o
作者
安文政
刘青
张超
邢微微
蔡贵玲
付文亮
徐东刚
AN Wen-zheng;LIU Qing;ZHANG Chao;XING Wei-wei;CAI Gui-ling;FU Wen-liang;XU Dong-gang(Institute of Military Cognition and Brain Sciences,Institute of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2023年第9期1-8,共8页
China Biotechnology
基金
国防科技工业局基础科研课题(JCKY2018548C001)资助项目。
关键词
贻贝
沙堡蠕虫
黏附肽
静电作用
复合凝聚
Mussel
Phragmatopoma californica
Adhesion peptide
Electrostatic action
Complexcondensation
