摘要
从大豆根际土壤中利用对羟基苯甲酸为唯一碳源,筛选获得6株具有对羟基苯甲酸降解及利用能力的根际促生菌,其降解能力强且降解底物多样。通过16S rRNA基因测序,确定根际促生菌菌株的分类地位,其中PA17为假单胞菌属(Pseudomonas),具有较好的应用前景。促生试验表明,PA17具有吲哚乙酸合成能力、固氮能力,同时具有解有机磷及无机磷的能力。发芽试验证明,PA17可促进大豆种子的萌发,并可显著促进大豆胚根的发育;盆栽试验表明PA17也可明显促进大豆幼苗的生长。该研究为生物方法缓解连作障碍提供高效降解菌株及理论依据。
Six rhizosphere promoting bacteria with the ability to degrade and utilize p-hydroxybenzoic acid were screened from soybean rhizosphere soil using p-hydroxybenzoic acid as the sole carbon source.These bacteria had strong degradation ability and diverse degradation substrates.By sequencing the 16S rRNA gene,the taxonomic status of rhizosphere growth promoting bacteria strains was determined,among which PA17 belongs to the genus Pseudomonas,which had good application prospects.The result of growth-promoting experiments indicated that PA17 showed the ability of IAA-producing,nitrogen-fixing and phosphate solubilization.Germination experiment and pot experiment showed that PA17 could significantly promote soybean seed germination,radicle and seeding growth.This study provides efficient degradation strains and theoretical basis for biological methods to alleviate continuous cropping obstacles.
作者
王丹丹
孙丽
于宏
崔世宇
庞诗琪
解志红
WANG Dan-dan;SUN Li;YU Hong(College of Resources and Environment,Shandong Agricultural University,Tai’an,Shandong 271000)
出处
《安徽农业科学》
CAS
2023年第20期11-14,21,共5页
Journal of Anhui Agricultural Sciences
基金
山东省自然科学基金项目(ZR2021QC175)
国家自然科学基金面上项目(32270065)
山东省重点研发计划项目(2021CXGC010804)。
关键词
对羟基苯甲酸
降解菌
连作障碍
根际促生菌
p-hydroxybenzoic acid
Degrading bacteria
Continuous cropping obstacle
Plant growth-promoting rhizobacteria