摘要
筛选羽衣甘蓝白色叶形成相关的关键基因和位点以及心叶颜色性状的遗传规律,以羽衣甘蓝红叶亲本WR、白叶亲本WB及其构建的F2分离群体为试验材料,从F2群体中挑选红叶和白叶植株各20株,分别构建2个DNA混合池,对子代混合池和亲本分别开展50×和20×覆盖深度的全基因组重测序,定位白叶性状关联区间,并根据基因注释信息预测候选基因。结果表明,重测序获得3 987 718个单核酸多态性(SNP)标记,获得位于第2、3和9染色体的8个显著关联区间,根据基因注释和功能分析,筛选到8个候选基因(Bol030253、Bol029431、Bol012077、Bol007709、Bol030318、Bol030235、Bol030286和Bol005195)。将8个基因确定为羽衣甘蓝白叶的候选基因,可能在羽衣甘蓝叶片颜色形成过程中起着重要作用。
This study is aimed to screen for key genes and loci associated with white leaf formation in kale(Brassica oleracea L.var.acephala DC)and the genetic patterns of heart leaf color traits.The kale red-leaf parent WR,the white-leaf parent WB and their constructed F2 segregating populations were used as test materials.Twenty red-leaf and white-leaf plants were selected from the F2 population,and two DNA mixing pools were constructed.Whole genome sequencing to offspring mixing pool and parents at 50x and 20x cover depth was conducted respectively,and the association interval of white leaf traits was located and candidate genes were predicted according to the gene annotation information.The results showed that re-sequencing 3987718 single nucleic acid polymorphism(SNP)markers were obtained via resequencing,8 significant association intervals on chromosomes 2,3,and 9 were obtained,and 8 candidate genes(Bol030253,Bol029431,Bol012077,Bol007709,Bol030318,Bol030235,Bol030286,and Bol005195)were obtained based on gene annotation and functional analysis.Identifying eight genes as candidate genes for white kale leaves may play an important role in the color formation of kale leaves.
作者
王腾辉
葛雯冬
罗雅方
范震宇
王玉书
WANG Teng-hui;GE Wen-dong;LUO Ya-fang;FAN Zhen-yu;WANG Yu-shu(College of Life Sciences,Agriculture and Forestry Qiqihar Universuty,Qiqihar 161006;Heilongjiang Provincial Key laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas,Qiqihar 161006)
出处
《生物技术通报》
CAS
CSCD
北大核心
2023年第9期176-182,共7页
Biotechnology Bulletin
基金
国家自然科学基金项目(31401908)
黑龙江省自然科学基金项目(C2016056)
黑龙江省教育厅基本科研业务专项(145209513)
黑龙江省普通高等学校青年创新人才培养计划(UNPYSCT-2017155)。
