摘要
为了解2021年我国猪圆环病毒2型(PCV2)流行情况,本试验于7个地区16个省采集1685份组织样品,采用实时荧光定量PCR检测方法检测PCV2,对其中45份阳性样品进行全基因组序列扩增和遗传演化分析。结果显示,被检样品PCV2总阳性率为9.85%(166/1685),场总阳性率为32.71%(35/107);各地区样品阳性率介于4.29%~19.00%,东北地区样品阳性率最高;各地区场阳性率介于6.90%~60.00%,华中地区场阳性率最高。PCV2a、PCV2b和PCV2d亚型分离毒株占比分别为31.11%、4.44%和64.44%,其中PCV2d亚型为优势流行株,未发现PCV2e和PCV2c亚型。有24株PCV2分离毒株的Cap蛋白在C末端有1个赖氨酸(K)延伸,另有1株PCV2分离毒株的Cap蛋白在C末端有1个蛋氨酸(M)延伸。结果表明,2021年我国PCV2感染范围广,污染程度高,且正在不断发生遗传变异,需要持续跟踪监测PCV2的流行情况,以便及时制定有效的防控措施。
In order to understand the prevalence of porcine circovirus type 2(PCV2)in China in 2021,a total of 1685 tissue samples were collected from 16 provinces in 7 regions in this study.Fluorescent quantitative PCR was used to detect PCV2,and 45 positive samples were selected for whole-genome sequencing and genetic evolutionary analysis.The results showed that the overall positive rate of PCV2 in the tested samples was 9.85%(166/1685),while the overall positive rate at the farms was 32.71%(35/107).The positive rate of the samples in different regions ranged from 4.29%to 19.00%,with the highest positive rate in northeast China.The positive rate of different farms in various regions ranged from 6.90%to 60.00%,with the highest positive rate in central China.The proportions of PCV2a,PCV2b and PCV2d subtypes were 31.11%,4.44%and 64.44%,respectively,with PCV2d subtype being the dominant prevalent strain.No PCV2e and PCV2c subtypes were detected.Among the isolated PCV2 strains,24 isolates had a lysine(K)extension at the C-terminal of Cap protein,and one isolate had a methionine(M)extension.This study indicates that PCV2 have a wide range of infections with high contamination rate in China in 2021,and ongoing genetic variations were observed.Continuous monitoring of PCV2 prevalence is necessary to develop timely and effective control measures.
作者
黄正波
崔进
张慧
尼博
刘爽
张锋
董雅琴
魏荣
代飞燕
吴发兴
HUANG Zheng-bo;CUI Jin;ZHANG Hui;NI Bo;LIU Shuang;ZHANG Feng;DONG Ya-qin;WEI Rong;DAI Fei-yan;WU Fa-xing(College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China;China Animal Health and Epidemiology Center,Qingdao 266032,China;Key Laboratory of Animal Biosafety Risk Prevention and Control(South),Ministry of Agriculture and Rural Affairs,Qingdao 266032,China)
出处
《中国兽医杂志》
CAS
北大核心
2023年第9期8-15,共8页
Chinese Journal of Veterinary Medicine
基金
“十四五”国家重点研发计划(2021YFD1800300)。
关键词
猪圆环病毒2型
流行病学调查
基因序列分析
porcine circovirus type 2
epidemiological investigation
gene sequence analysis
