摘要
【目的】为了寻找有效防治朱砂叶螨的生物学方法。【方法】克隆朱砂叶螨V-ATPaseH基因,对该基因序列进行生物学特征分析;采用实时荧光定量PCR技术对V-ATPaseH进行不同时期表达特征分析。【结果】成功克隆V-ATPaseH基因(GenBank登陆号:OQ834270),基因全长1540 bp,阅读开放框为1475 bp,编码491个氨基酸;V-ATPaseH在朱砂叶螨的卵、幼螨、若螨、成螨4个时期均有表达。其中以朱砂叶螨成螨时期表达量最高,其次是若螨、卵,幼螨时期表达量最低。【结论】克隆得到朱砂叶螨V-ATPaseH基因,明确其不同时期的表达特征,为今后研究以V-ATP酶为靶标的新型生物农药杀螨剂研制奠定基础。
[Objective]Search for an effective biological control method for Tetranychus cinnabarinus.[Methods]The V-ATPaseH gene was cloned and its gene sequence was analyzed;real-time quantitative fluorescence PCR was used to characterize the expression of V-ATPaseH.[Results]The V-ATPaseH gene(GenBank accession number:OQ834270)was successfully cloned with a total length of 1540 bp and an open frame of 1475 bp,encoding 491 amino acids.The expression of V-ATPaseH was highest in the adult mite stage,followed by the nymph and egg stages,and lowest in the larva stage.[Conclusion]The V-ATPaseH gene was cloned and its expression characteristics at various stages were clarified,which may provide a basis for the development of new biopesticidal acaricides targeting the V-ATPase in the future.
作者
王谦稳
陈耔霖
李安琪
任争光
卜春亚
WANG Qianwen;CHEN Zilin;LI Anqi;REN Zhengguang;BU Chunya(College of Biological and Resource Environment/Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs,Beijing University of Agriculture,Beijing 102206,China)
出处
《北京农学院学报》
2023年第4期15-19,共5页
Journal of Beijing University of Agriculture
基金
国家自然科学基金资助项目(31670648)
北京市自然科学基金资助项目(6212004)。
关键词
朱砂叶螨
V-ATP酶
基因克隆
荧光定量PCR
Tetranychus cinnabarinus
V-ATPase
gene cloning
fluorescence quantitative PCR
