摘要
采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UHPLC-Q/Orbitrap HRMS)技术对西藏3个地区的41批甘青青兰样本进行分析,通过Compound Discoverer 3.2软件对化学成分进行鉴定,并利用多元统计学分析筛选出不同地区样本之间的差异性成分。在甘青青兰样本中共鉴定73种化合物,包括41种黄酮类、15种有机酸类、8种氨基酸类以及9种其他类化合物。经多元统计学分析,山南市样本(SN)与拉萨市样本(LS)之间有11种差异化合物,山南市样本(SN)与昌都市样本(CD)之间有18种差异化合物,拉萨市样本(LS)与昌都市样本(CD)之间有21种差异化合物(VIP>1,P<0.05)。大多数差异化合物为黄酮类及有机酸类化合物。绿原酸在3组样本间的含量差异最大,在CD组含量最高(约为11.85 mg/g),LS组次之(约为7.85 mg/g),SN组最低(约为5.30 mg/g),这与甘青青兰样本的叶子数量和粉末颜色有一定的相关性。结合各地区地势及气候推测光照日长及日温差对甘青青兰化学成分有影响。综上所述,推测地理环境的差异对甘青青兰的性状和化学成分具有影响。研究结果为甘青青兰质量控制及后续开发利用提供了理论基础。
This study used ultra-high performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrometry(UHPLC-Q/Orbitrap HRMS)technology to analyze 41 batches of Ganq-ingqinglan samples from three regions in Tibet.Compound Discoverer 3.2 software was utilized to identify the chemical components,and differential compounds between different regions were screened through multivariate statistical analysis.A total of 73 compounds were identified in the Gan-qingqinglan samples,including 41 flavonoids,15 organic acids,8 amino acids,and 9 other com-pounds.Through multivariate statistical analysis,11 differential compounds were found between samples from Shannan City(SN)and Lhasa City(LS),18 differential compounds between SN and Qamdo City(CD),and 21 differential compounds between LS and CD(VIP>1,P<0.05).The ma-jority of differential compounds were flavonoids and organic acids.Chlorogenic acid exhibited the largest difference among the three groups,with the highest content found in the CD group(approxi-mately 11.85 mg/g),followed by the LS group(approximately 7.85 mg/g),and the lowest content in the SN group(approximately 5.30 mg/g).This correlated with the leaf quantity and powder color of the Ganqingqinglan samples.Considering the topography and climate of each region,it is speculated that sunlight duration and diurnal temperature range have an influence on the chemical composition of Ganqingqinglan.In conclusion,geographical environmental differences are believed to have an im-pact on the characteristics and chemical composition of Ganqingqinglan.These research findings pro-vide a theoretical basis for quality control and subsequent rational development and utilization of Ganqingqinglan.
作者
段婷引
达娃卓玛
兰钧
李雪敏
张梦姣
周燕
邓放
DUAN Ting-yin;DAWA Droma;LAN Jun;LI Xue-min;ZHANG Meng-jiao;ZHOU Yan;DENG Fang(Stated Key Laboratory of Chengdu Southwest Chinese Medicine Resources,School of Pharmacy,University of Traditional Chinese Medicine,Chengdu 611137,China;Key Laboratory of Quality Control of Traditional Chinese Medicine(Tibetan Medicine),Tibet Autonomous Region Institute for Food and Drug Control,Lasa850000,China;Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2023年第10期1265-1271,共7页
Journal of Instrumental Analysis
基金
西藏自治区科技计划项目(XZ202101ZD0024G)。
