摘要
目的研究自组装颗粒物理性征及其对BRL-3A细胞毒性作用及氧化应激水平的影响。方法将壳聚糖制备成纳米颗粒储备液(10μg/ml)并行倍比稀释后检测其对BRL-3A细胞活力影响,以壳聚糖纳米颗粒为载体与齐墩果酸自组装,同时观察齐墩果酸自组装后物理性征变化。将BRL-3A细胞分为齐墩果酸(OA)组及齐墩果酸-壳聚糖纳米颗粒(nanoOA)组,分别施加0,6.25,12.5,25,50,100μg/ml OA和nanoOA。检测干预3,6,12,24 h后细胞相对活力变化,及各组作用24 h后细胞活性氧水平(ROS)和丙二醛(MDA)含量变化。结果壳聚糖纳米颗粒对BRL-3A细胞相对活力无影响(P<0.05),自组装后可明显提高OA溶解度。与0μg/ml组相比,高浓度OA组(50,100μg/ml)BRL-3A细胞相对活力明显降低(P<0.05),细胞ROS和MDA水平明显增加(P<0.05),且呈时间依赖性。而不同浓度nanoOA作用后BRL-3A细胞相对活力没有明显改变,ROS水平增加(P<0.05),MDA水平未见明显增加(P>0.05),且除0μg/ml外,与同浓度OA组相比ROS、MDA水平较低(P<0.05)。结论壳聚糖纳米颗粒具有生物安全性,自组装后可改善OA的水溶性,并减少OA对细胞活力和氧化应激指标的影响。
Objective To study the physical properties of self-assembled particles,and explore its effects on the toxicity to BRL-3A cells and oxidative stress.Methods Chitosan nanopaticle stock solution(10μg/ml)was prepared and diluted,and then the effect of self-assembled particles on BRL-3A cell viability was detected.Simultaneously,the physical properties of oleanolic acid after self-assembly were observed.BRL-3A cells were divided into oleanolic acid(OA)group and oleanolic acid-chitosan nanoparticles(nanoOA)group,and then treated with different concentrations(0,6.25,12.5,25,50,100μg/ml)of OA and nanoOA,respectively.The relative activity of BRL-3A cells was detected after intervention for 3,6,12,24 h,and the level of reactive oxygen species(ROS)and the content of malondialdehyde(MDA)in cells were measured at 24 h.Results Chitosan nanoparticles showed no effect on BRL-3A cells viability(P<0.05),and the solubility of OA was significantly improved after self-assembly.Compared with 0μg/ml group,the relative activities of BRL-3A cells were decreased significantly in 50,100μg/ml OA groups(P<0.05),while the levels of ROS and MDA in cells were increased time-dependently(P<0.05).The relative activity of BRL-3A cells did not change significantly after treated with different concentrations of nanoOA,ROS level was increased(P<0.05),while MDA level showed no significant increase(P>0.05).Moreover,the levels of ROS and MDA were lower in nanoOA group at 6.25,12.5,25,50,100μg/ml than those in OA group(P<0.05).Conclusion Chitosan nanoparticles have good biosafety,and can improve the water solubility of OA after self-assembly,and reduce the impact of OA on cell viability and oxidative stress indicators.
作者
金磊
王帅
于卫华
刘江正
刘瑞
孔德钦
姬秋和
李文丽
JIN Lei;WANG Shuai;YU Weihua;LIU Jiangzheng;LIU Rui;KONG Deqin;JI Qiuhe;LI Wenli(Department of Military Toxicology and Chemical Defense Medicine,School of Military Preventive Medicine,Ministry of Education Key Laboratory of Hazard Assessment and Control in Special Operational Environment,Shaanxi Provincial Key Laboratory of Free Radical Biology and Medicine,Air Force Medical University,Xi’an 710032,China;Department of Endocrinology,901th Hospital of PLA Joint Logistic Support Force;Department of Pathology,Ninth People’s Hospital of Wuxi;Department of Endocrinology,First Affiliated Hospital of Air Force Medical University)
出处
《山西医科大学学报》
CAS
2023年第9期1268-1273,共6页
Journal of Shanxi Medical University
基金
国家自然科学基金面上项目(82173544)
陕西省自然重点研发计划项目(2022ZDLSF07-11)。
关键词
壳聚糖
齐墩果酸
氧化应激
活性氧
纳米化
chitosan
oleanolic acid
oxidative stress
reactive oxygen species
nanocrystallization
