摘要
以越南石斛(Dendrobium vietnamense Aver.)为植物材料,硅酸钙为外源添加物,筛选最适合添加到越南石斛的原球茎诱导培养基、壮苗生根培养基中的硅酸钙浓度。结果表明,最适合添加到越南石斛原球茎诱导培养基的硅酸钙浓度为0.2 g/L,原球茎诱导率为71.43%;对越南石斛组培苗的防褐化效果最佳的硅酸钙浓度为0.5 g/L;株高生长最多的最佳硅酸钙浓度为0.4 g/L;枝条数最多的硅酸钙浓度为0.3 g/L;叶片数最多的硅酸钙浓度为0.3 g/L;当硅酸钙浓度为0.3~0.5 g/L时,越南石斛生根效果较佳。
Using Dendrobium vietnamense as plant material and calcium silicate as the exogenous additive,the calcium silicate concentration that was most suitable for adding to protocorm induction medium and strong seedling rooting medium of Dendrobium vietnamense was selected.The results showed that the most suitable calcium silicate concentration added to the protocorm induction medium of Dendrobium vietnamense was 0.2 g/L,and the protocorm induction rate was 71.43%;the best anti-browning effect of tissue culture seedlings of Dendrobium vietnamense could be obtained when the calcium silicate concentration was 0.5 g/L;the optimum calcium silicate concentration for plant height growth was 0.4 g/L;the calcium silicate concentration with the largest number of branches was 0.3 g/L;the concentration of calcium silicate with the largest number of leaves was 0.3 g/L;when the concentration of calcium silicate was 0.3~0.5 g/L,the rooting effect of Dendrobium vietnamense was better.
作者
王义琴
孙博
何玲
师凯辉
黄鑫
王冬梅
陈宇
臧睿
和凤美
WANG Yi-qin;SUN Bo;HE Ling;SHI Kai-hui;HUANG Xin;WANG Dong-mei;CHEN Yu;ZANG Rui;HE Feng-mei(College of Landscape and Horticulture,Yunnan Agricultural University,Kunming 650201,China)
出处
《湖北农业科学》
2023年第9期78-80,123,共4页
Hubei Agricultural Sciences
基金
国家自然科学基金项目(31760591)
晋宁区花卉产业科技特派团项目(202204BI090022)
云南农业大学学生科技创新创业行动基金项目(2021ZKX072)。