摘要
BBXs(B-box)转录因子家族在植物生长发育和胁迫反应中发挥重要作用。本研究利用生物信息学手段在毛果杨(Populus trichocarpa)基因组筛选并鉴定出28个PtBBXs基因家族成员,对该家族成员进行结构和表达分析表明,PtBBXs基因不均匀地分布于15条染色体上,其编码的氨基酸残基数介于184-514,全部为酸性蛋白质。依据基因结构和蛋白质保守基序,可将其分为5个组(A-E),全基因组加倍事件是PtBBXs家族基因数量扩增的主要方式。PtBBX家族基因的启动子区域包含大量激素(脱落酸、赤霉素,和水杨酸等)与非生物胁迫(干旱、低温)响应相关的元件,大多数PtBBXs基因在叶片中高表达,而少量PtBBXs基因(PtBBX5/11/12/28)在根或花序中高表达。转录组数据分析表明,PtBBXs在响应非生物胁迫过程中分工不同,组A和组D的多数成员响应了茉莉酸、水杨酸和低温处理,组B(PtBBX16/19)和组C(PtBBX1/2)基因同时响应盐和干旱胁迫。这些结果为今后进一步挖掘PtBBX基因家族成员功能,以及创制林木抗逆新种质,提供了重要的理论依据。
The BBX(B-box)family of transcription factors plays an important role in plant growth and development and stress response.In this study,28 PtBBX gene family members were identified in the genome of Populus trichocarpa.Structural and expression analysis showed that PtBBX genes were distributed unevenly on 15 chromosomes and encoded peptides ranging from 184 to 514 aa that were all acidic.The promoter regions of PtBBX genes containd a large number of elements related to hormone(abscisic acid,gibberellin,and salicylic acid,etc.)and abiotic stress responses(drought,low temperature).Most PtBBX genes were highly expressed in leaves.While a few PtBBX genes(PtBBX5/11/12/28)were highly expressed in roots or inflorescences.Analysis of transcriptome data showed that PtBBXs functioned differentially in response to abiotic stresses.Most members of group A and D responded to jasmonic acid,salicylic acid and low temperature treatments,and group B(PtBBX16/19)and group C(PtBBX1/2)genes responded to both salt and drought stresses.These results provided an important theoretical basis for further exploring the functions of PtBBX gene family members and creating new germplasm for stress resistance in forest trees in the future.
作者
齐小坤
孟祥熹
赵志明
袁梦婷
杨志慧
武江昊
王进茂
QI Xiaokun;MENG Xiangxi;ZHAO Zhiming;YUAN Mengting;YANG Zhihui;WU Jianghao;WANG Jinmao(College of Forestry/Key Laboratory of Forest Germplasm Resources and Forest Protection,Hebei Agricultural University,Baoding 071000,China)
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2023年第5期41-49,共9页
Journal of Hebei Agricultural University
基金
河北省重点研发计划项目(21326301D).
关键词
BBX基因家族
毛果杨
生物信息学
转录响应
BBX gene family
Populus trichocarpa
bioinformatics
transcriptional response
