摘要
目的分析丹参酮ⅡA通过调控核转录因子(NF)-κB信号通路对胶质瘤的作用机制。方法体外培养人神经胶质瘤U87细胞,分别加入0、5、10 mg/kg的丹参酮ⅡA培养72 h(即0 mg/kg组、5 mg/kg组、10 mg/kg组),并在培养24、48、72 h时采用MTT法检测细胞增殖,流式细胞术检测细胞凋亡,Transwells小室检测细胞迁移和侵袭情况,并比较各组细胞培养72 h后的细胞各炎症因子水平、Toll样受体4(TLR4)、核转录因子-κB(NF-κB)蛋白及mRNA水平。结果使用不同浓度丹参酮ⅡA对人神经胶质瘤U87细胞处理72 h,随处理时间的延长各组细胞吸光度值均升高,培养24、48和72 h时,5 mg/kg组、10 mg/kg组的细胞吸光度值均低于0 mg/kg组,且10 mg/kg组低于5 mg/kg组,差异均有统计学意义(P<0.05),呈剂量-反应关系。随处理时间的延长各组细胞凋亡率均升高,培养24、48、72 h时,5 mg/kg组、10 mg/kg组的细胞凋亡率均高于0 mg/kg组,且10 mg/kg组高于5 mg/kg组,差异均有统计学意义(P<0.05),呈剂量-反应关系。随处理时间的延长各组细胞迁移个数均增多,培养24、48、72 h时,5 mg/kg组、10 mg/kg组的细胞迁移个数均少于0 mg/kg组,且10 mg/kg组少于5 mg/kg组,差异均有统计学意义(P<0.05),呈剂量-反应关系。随处理时间的延长各组细胞侵袭个数均增多,培养24、48、72 h时,5 mg/kg组、10 mg/kg组的细胞侵袭个数均少于0 mg/kg组,且10 mg/kg组少于5 mg/kg组,差异均有统计学意义(P<0.05),呈剂量-反应关系。使用不同浓度丹参酮ⅡA对人神经胶质瘤U87细胞处理72 h,5 mg/kg组、10 mg/kg组的TNF-α、IL-1β、IL-6水平均低于0 mg/kg组,且10 mg/kg组低于5 mg/kg组,差异均有统计学意义(P<0.05),呈剂量-反应关系。5 mg/kg组、10 mg/kg组的TLR4蛋白、NF-κB p50蛋白、TLR mRNA、NF-κB mRNA水平均低于0 mg/kg组,且10 mg/kg组低于5 mg/kg组,差异均有统计学意义(P<0.05),呈剂量-反应关系。结论丹参酮ⅡA可通过调控NF-κB信号通路�
Objective To analyze the mechanism of tanshinoneⅡA by regulating the nuclear transcription factor(NF)-κB signaling pathway on glioma.Methods Human glioma U87 cells were cultured in vitro,and tanshinoneⅡA(0 mg/kg,5 mg/kg,10 mg/kg)was added to culture for 72 h.MTT method was used to detect cell proliferation,flow cytometry was used to detect cell apoptosis,Transwells cell was used to detect cell migration and invasion,and the levels of inflammatory factors,TLR4,NF-κB protein and mRNA in each group after 72 h culture were compared.Results Human glioma U87 cells were treated with different concentrations of tanshinoneⅡA for 72 h,and the cell absorbance values of each group increased with the prolongation of treatment time.When cultured for 24,48 and 72 h,the cell absorbance values of the 5 mg/kg group and the 10 mg/kg group were lower than the 0 mg/kg group,and the 10 mg/kg group was lower than the 5 mg/kg group,the differences were statistically significant(P<0.05),showing a dose response.With the prolongation of treatment time,the apoptosis rate of cells in all groups increased.At 24,48 and 72 h of culture,the apoptosis rate of cells in the 5 mg/kg group and the 10 mg/kg group was higher than that in the 0 mg/kg group,and that in the 10 mg/kg group was higher than that in the 5 mg/kg group,the differences were statistically significant(P<0.05),showing a dose response.With the prolongation of treatment time,the number of cell migration in each group increased.When cultured for 24,48 and 72 h,the number of cell migration in the 5 mg/kg group and the 10 mg/kg group was less than that in the 0 mg/kg group,and the number of cell migration in the 10 mg/kg group was less than that in the 5 mg/kg group,the differences were statistically significant(P<0.05),showing a dose response.With the prolongation of treatment time,the number of cell invasion in each group increased.When cultured for 24,48 h and 72 h,the number of cell invasion in the 5 mg/kg group and the 10 mg/kg group was less than that in the 0 mg/kg group,
作者
隋航
栾雷
曹志刚
李茂雷
SUI Hang;LUAN Lei;CAO Zhi-gang(Department of Neurosurgery,Chengyang District People's Hospital,Qingdao Shandong 266109,China;Department of Health Management,Chengyang District People's Hospital,Qingdao Shandong 266109,China)
出处
《临床和实验医学杂志》
2023年第18期1908-1912,共5页
Journal of Clinical and Experimental Medicine
基金
山东省自然科学基金青年项目(编号:ZR2020QH104)。
