摘要
目的探讨同源框蛋白13(HOX13)与糖尿病足(DF)患者临床特征的相关性及对细胞增殖、凋亡及炎症的影响。方法选取2018年1月—2021年1月中国中医科学院广安门医院南区接诊的糖尿病足患者60例作为糖尿病足组,选择同期因外伤行清创手术的2型糖尿病非病足患者19例作为糖尿病非病足组,并选择同期因外伤在我院接受清创手术的无糖尿病病史且无创面感染的患者60例作为对照组。对比分析3组临床特征及其HOX13相关性。3组间HOX13表达采用免疫组化法;HOX13蛋白表达使用Western blot检测。构建体外细胞模型,分为敲除组(转染敲除HOX13质粒)、HOX13过表达组(转染过表达HOX13质粒)、空白组(未做任何处理)。检测细胞增殖能力通过CCK-8和edu实验;细胞凋亡检测采用流式细胞仪。结果糖尿病非病足组年龄、病程、白细胞计数、纤维蛋白原(FG)、糖化血红蛋白(HbA1c)、肿瘤坏死因子β(TNF-β)、超敏C反应蛋白(hs-CRP)、白介素(1L)-6、1L-4显著低于糖尿病足组(P<0.05),对照组年龄、病程、白细胞计数、FG、HbA1c、TNF-β、hs-CRP、1L-6、1L-4显著低于糖尿病非病足组(P<0.05)。踝肱指数、血红蛋白、白蛋白在糖尿病非病足组显著高于糖尿病足组(P<0.05)。HOX13的阳性表达与年龄、病程、白细胞计数、FG、HbA1c、TNF-β、hs-CRP、1L-6、1L-4呈负相关(P<0.05);踝肱指数及白蛋白与HOX13呈正相关(P<0.05)。免疫组化结果显示,相较于糖尿病非病足组与对照组,糖尿病足组的HOX13表达显著降低,较于空白组,HOX13敲除组TNF-β、hs-CRP、1L-6、1L-4显著偏高,过表达组显著降低(均P<0.05)。较于空白组,敲除组Caspase-3、Caspase-9、Bax蛋白表达显著偏高,过表达组显著偏低(均P<0.05),HOX13敲除组Bcl-2蛋白表达显著偏低,过表达组显著偏高(P<0.05)。流式细胞仪检测细胞凋亡结果表明HOX13敲除明显增加细胞凋亡(P<0.05)。CCK-8实验结果表明,过表达组细胞�
Objective To investigate the effect of Homeobox(HOX)protein 13 on cell proliferation and apoptosis in patients with diabetic foot.Methods 79 patients were selected as the research object,which were divided into diabetes foot group(n=60),diabetes non diseased foot group(n=19),and healthy control group(n=60).The clinical characteristics and correlation of HOX13 among the three groups were compared and analyzed.The expression of HOX13 among the three groups was determined by immunohistochemistry.Western blot was used for HOX13 protein expression.Cell proliferation was detected by CCK-8 and EDU assay.Cell apoptosis was detected by flow cytometry.In vitro cell models were constructed and divided into HOX13 knockout group(transfected with HOX13 plasmid),HOX13 overexpression group(transfected with HOX13 plasmid),and blank group(without any treatment).Results Age,disease course,white blood cell count,FG,HbA1c,TNF-β,hs-CRP,1L-6,1L-4 in non-diseased foot group were significantly lower than those in diabetic foot group(P<0.05).Age,disease course,white blood cell count,FG,HbA1c,TNF-β,hs-CRP,1L-6,1L-4 in healthy control group were significantly lower than those in non-diseased foot group(P<0.05).The ankle-brachial index,hemoglobin and albumin in non-diseased foot group were significantly higher than those in diabetic foot group(P<0.05).The positive expression of HOX13 was negatively correlated with age,disease course,white blood cell count,FG,HbA1c,TNF-β,hs-CRP,1L-6,1L-4(P<0.05).Ankle-brachial index and albumin were positively correlated with HOX13(P<0.05).Immunohistochemical results showed that HOX13 expression was significantly decreased in diabetic foot group compared with non-diseased foot group and healthy control group.Compared with blank group,TNF-β,hs-CRP,1L-6 and 1L-4 were significantly higher in HOX13 knockout group(P<0.05),while significantly lower in overexpression group(P<0.05).Compared with blank group,the protein expressions of Caspase-3,Caspase-9 and Bax were significantly higher in HOX13 knockout group,an
作者
刘宇彪
周花玩
鞠上
LIU Yubiao;ZHOU Huawan;JU Shang(Department of Surgery,South Area of Guang’anmen Hospital South Campus,China Academy of Chinese Medical Sciences,Beijing 102600,China;Tiancheng Jinxiang Traditional Chinese Medicine Clinic,Beijing 102600,China;Department of Peripheral Blood Vessels,Dongzhimen Hospital,Beijing University of Traditional Chinese Medicine,Beijing 102600,China)
出处
《西部医学》
2023年第10期1425-1432,共8页
Medical Journal of West China
基金
国家重点研发计划项目(2019YFC1709302)。
