摘要
目的以红花-桃仁药对汤液中相态差异为基础,探究煎煮过程对该药对药效物质的传递作用。方法采用超速离心和透析技术对红花-桃仁药对汤液进行相态拆分,使用马尔文粒径仪、透射电子显微镜对各相态的粒径、ζ电位、微观形态进行表征,选用气相色谱法、紫外分光光度法、HPLC法对各相态的脂肪酸、蛋白、糖类以及红花有效成分羟基红花黄色素A(hydroxysafflor yellow A,HSYA)、脱水红花黄色素B(anhydrosafflor yellow B,AHSYB)的含量进行测定;拆分单煎液、分煎合并液及共煎液的相态,并比较不同药液中同级相态的物质组成或微观形态的差异,考察煎煮方式对相态形成的影响。结果红花-桃仁药对汤液中分离得到了1个亲油型相态②和6个亲水型相态①③④⑤⑥⑧;亲油型相态②由棕榈酸、棕榈油酸、十七烷酸、硬脂酸、油酸、亚油酸、花生四烯酸、二十烷酸共8种脂肪酸组成;亲水型相态粒径在20~1000 nm,主要为混悬相态与胶体相态,糖类、蛋白是亲水型相态的重要组成物质,HSYA、AHSYB主要分布于亲水型相态③⑥⑧中。共煎煮可形成亲油型相态,其脂肪酸组成与桃仁油相近,单煎液、分煎合并液及共煎液中亲水型相态⑥⑧粒子的形态有一定差异,共煎液中相态⑥⑧粒子的粒径均一性、稳定性最佳。结论物相形态是红花-桃仁药对药效物质传递的重要形式,煎煮过程特别是共煎煮,是该药对有效相态形成的重要步骤。
Objective To explore the delivering effects of decocting process on the pharmacodynamic substances in Carthami Flos-Persicae Semen herb pair based on the phase state differences.Methods The phase states of the co-decoction were separated using ultracentrifugation and dialysis,and the particle size,ζpotential,and microscopic morphology of each phase state were characterized by Zetasizer Nano ZS instrument and transmission electron microscope.The content of fatty acids,proteins,saccharine,hydroxysafflor yellow A(HSYA)and anhydrosafflor yellow B(AHSYB)were determined by gas chromatography,UV spectrophotometry and HPLC.The effects of decoction modes on phase states formation were investigated by splitting the phase states in single-,merged-and co-decoction and then comparing the composition or microscopic forms of phase states in different decoctions.Results One oleophilic phase state②and six hydrophilic phase states including①,③,④,⑤,⑥,and⑧were isolated from Carthami Flos-Persicae Semen herb pair decoction;The oleophilic phase state consisted of eight fatty acids including palmitic acid,palmitoleic acid,margaric acid,stearic acid,oleic acid,linoleic acid,arachidonic acid,and arachidic acid and the hydrophilic phases were mainly miscible or colloidal phase states with the particle size ranging from 20 to 1000 nm.Both saccharine and proteins are important constituents of the hydrophilic phases,and HSYA,AHSYB are predominantly distributed in the hydrophilic phases③,⑥and⑧.Co-decocting can form oleophilic phase state,whose fatty acid composition is similar to that of peach kernel oil.The microscopic morphology of hydrophilic phases⑥and⑧in single-,merged-and co-decoction are slightly different,and phases⑥and⑧in co-decoction present the good particle size uniformity and stability.Conclusion The phase state is an important form for delivering the pharmacodynamic substances in Carthami Flos-Persicae Semen herb pair,and the decocting process,especially the co-decocting,is the essential procedure fo
作者
陶春晓
叶泰玮
李敏
张芳
谢燕
TAO Chun-xiao;YE Tai-wei;LI Min;ZHANG Fang;XIE Yan(School of Public Health,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;The Office of Experts Committee,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Innovative Research Institute of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《中草药》
CAS
CSCD
北大核心
2023年第17期5550-5559,共10页
Chinese Traditional and Herbal Drugs
基金
上海市卫生健康学科带头人项目(2022XD026)。
关键词
中药药对
红花
桃仁
煎煮过程
相态
超速离心
透析技术
脂肪酸
蛋白
糖类
羟基红花黄色素A
脱水红花黄色素B
药效物质传递
herb pairs
Carthami Flos
Persicae Semen
decocting process
phase state
ultracentrifugation
microdialysis technique
fatty acids
proteins
saccharine
hydroxysafflor yellow A
anhydrosafflor yellow B
pharmacodynamic substance delivery
