摘要
目前脂肪酶在食品工业中常用于油脂的改性,例如酯化和酯交换等。为了提高脂肪酶在使用过程中的化学稳定性、热稳定性以及重复利用性,使用戊二醛(glutaraldehyde,GDA)作为交联剂将脂肪酶与乙酰化花生球蛋白颗粒(acetylated arachin particles,AAPs)进行交联固定,并对蛋白载酶颗粒(lipase-AAPs)的固载效率和比活性进行优化。结果发现,当丁二酸酐添加量为蛋白质干基质量的30%(乙酰化程度为41.71%±0.01%)、GDA浓度为1.0%、交联时间1 h、浓度为10%以及脂肪酶与颗粒比例为1∶4时,lipase-AAPs最佳固载效率和比活性分别为12.95%±0.03%和1.74±0.07 U·mg^(-1)。使用扫描电子显微镜(scanning electron microscopy,SEM)观察固载前后的脂肪酶微观形态;使用傅里叶变换红外光谱(fourier transform infrared spectroscopy,FTIR)对脂肪酶与颗粒相互作用进行了表征。研究结果旨在提供一种蛋白载酶颗粒的制备方法,为天然有机大分子作为脂肪酶载体提供理论支撑和技术支持,避免无机与有机合成颗粒载酶带来的安全性问题。
Lipases are currently commonly used in the food industry for the modification of oils and fats,such as esterification and transesterification.In order to improve the chemical stability,thermal stability,and reusability of lipases,glutaraldehyde(GDA)was used as a crosslinking agent to crosslink lipase with acetylated arachin particles(AAPs).And the immobilization efficiency and specific activity of protein carrier enzyme particles(lipase-AAPs)were optimized.The results showed that when the addition amount of succinic anhydride was 30%of the dry base mass of protein(the degree of acetylation was 41.71%±0.01%),the GDA concentration was 1.0%,the crosslinking time was 1 h,the lipase concentration was 10%,and the lipase to particle ratio was 1∶4,the optimal immobilization efficiency and specific activity of lipase-AAPs were 12.95%±0.03%and 1.74±0.07 U·mg^(-1),respectively.Micromorphological observation of lipase before and after crosslinking was performed using scanning electron microscopy(SEM).Characterization of interactions between protein and lipases was measured by fourier transform infrared spectroscopy(FTIR).In this paper,a method for the preparation of protein-loaded enzyme particles was provided,which was expected to provide theoretical and technical support for natural organic macromolecules as lipase carriers and avoid the safety problems caused by inorganic and organic synthetic particles.
作者
李薇
石爱民
焦博
王强
LI Wei;SHI Aimin;JIAO Bo;WANG Qiang(Key Laboratory of Agro-products Processing,Ministry of Agriculture and Rural Affairs,Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《生物技术进展》
2023年第5期771-778,共8页
Current Biotechnology
基金
国家重点研发计划项目(2022YFF1101400)。
