摘要
目的建立罗汉果超高效液相色谱(UPLC)指纹图谱及多指标成分含量测定方法,为完善罗汉果药材的质量评价提供依据。方法采用Waters Cortecs T3 C18色谱柱(2.1 mm×100 mm,1.6μm),流动相为乙腈-0.1%磷酸水,梯度洗脱,流速0.30 mL/min,检测波长0~25 min为300 nm、25.1~40 min为203 nm,柱温43℃,进样量1μL;利用《中药色谱指纹图谱相似度评价系统》(2012版)对15批罗汉果药材UPLC指纹图谱进行相似度分析;采用SPSS25.0和SIMCA14.1软件对罗汉果药材进行化学计量学分析;测定罗汉果药材中多指标成分的含量。结果15批罗汉果药材指纹图谱有16个共有峰,指认其中4种成分;聚类分析和主成分分析均将15批罗汉果药材分为两类,其中广西柳州样品为一类,广西桂林样品为一类;以变量重要性投影(VIP)值>1.0为条件,共筛选出6个差异性标志物,按VIP值排序,峰13>峰9>峰5>峰7>峰15(罗汉果皂苷Ⅴ)>峰2(5-羟甲基糠醛);广西柳州样品5-羟甲基糠醛含量高于广西桂林样品,罗汉果皂苷Ⅴ含量则略低。结论本研究建立的UPLC指纹图谱方法和含量测定方法可行,可为罗汉果质量评价提供依据。
Objective To establish the UPLC fingerprint and multi-index component content determination method of Siraitiae Fructus;To provide a basis for optimize the quality evaluation of Siraitiae Fructus.Methods Waters Cortecs T3 C18 column(2.1 mm×100 mm,1.6μm)was used.The mobile phase was acetonitrile-0.1%phosphoric acid water with gradient elution.The flow rate was 0.30 mL per minute.The detection wavelength was 300 nm in 0–25 min and 203 nm in 25.1–40 min.The column temperature was 43°C and the injection volume was 1μL.The UPLC fingerprints of 15 batches of Siraitiae Fructus were analyzed by the Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine(2012 Edition);the Chemometrics analysis of Siraitiae Fructus was carried out by SPSS 25.0 and SIMCA 14.1 software;multi index components in Siraitiae Fructus were determined.Results The results showed that there were 16 common peaks in the fingerprints of 15 batches of Siraitiae Fructus,and 4 components were identified.The 15 batches of Siraitiae Fructus were divided into two categories by clustering analysis and PCA,among which Liuzhou in Guangxi was classified into one category and Guilin in Guangxi was classified into one category.The VIP value>1.0 was used as the screening condition to screen the differential markers.A total of 6 differential markers were found.The VIP value was ranked as peak 13>peak 9>peak 5>peak 7>peak 15(mogroside V)>peak 2(5-hydroxymethylfurfural);the results of content determination showed that the content of 5-hydroxymethylfurfural in Siraitiae Fructus from Liuzhou,Guangxi was higher than that from Guilin,while the content of mogroside V was slightly lower than that from Guilin.Conclusion The UPLC fingerprint method and content determination method established in this study are feasible and can provide a basis for the quality evaluation of Siraitiae Fructus.
作者
张芳平
刘晓霞
位翠杰
冯涌微
何民友
夏天睿
陈向东
孙冬梅
李振雨
ZHANG Fangping;LIU Xiaoxia;WEI Cuijie;FENG Yongwei;HE Minyou;XIA Tianrui;CHEN Xiangdong;SUN Dongmei;LI Zhenyu(Guangdong Yifang Pharmaceutical Co.Ltd.,Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula,Foshan 528244,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2023年第10期139-144,共6页
Chinese Journal of Information on Traditional Chinese Medicine
基金
中华人民共和国工业和信息化部2022年产业技术基础公共服务平台项目(2022-230-221)。
