摘要
目的观察循经通络推拿手法对乳腺增生(MGH)模型大鼠的治疗作用,并对比单纯推拿手法、药物治疗、手法联合药物治疗的疗效,探讨其调节大鼠性激素水平发挥治疗作用的机制。方法将50只SD大鼠随机分为对照组、模型组、手法组、药物组、药物加手法组共5组,每组10只。除对照组外,其余各组连续肌肉注射苯甲酸雌二醇(0.5 mL/kg)25 d,随后连续肌肉注射黄体酮(5 mg/kg)5 d建立乳腺增生模型。手法组予以循经通络推拿手法治疗,药物组给予三苯氧胺(0.5 mg/kg)灌胃治疗,药物加手法组同时给予循经通络推拿和药物灌胃,连续治疗30 d。观察各组大鼠一般体征,测量大鼠第2对左右乳头直径和乳头高度,HE染色观察乳腺组织病理变化,ELISA法检测血清中雌二醇(E_(2))和孕酮(P)含量,Western-blot检测乳腺组织中雌激素受体α(ERα)、孕激素受体(PR)的蛋白表达。结果与空白组比较,模型组大鼠乳头高度和直径显著增大(P<0.05),乳腺组织增生病变明显,血清中E_(2)含量显著升高,P含量显著降低(P<0.05),明显上调了乳腺组织中ERα的蛋白表达,下调了PR的表达(P<0.05)。手法组、药物组、药物加手法组大鼠乳头直径、乳头高度均较模型组显著减小(P<0.05);药物加手法组大鼠乳腺组织形态明显改善,增生程度明显减轻,手法组和药物组大鼠乳腺组织病理形态较模型组改善不明显;药物组和药物加手法组大鼠E_(2)含量及ERα的蛋白表达明显降低,P含量和PR的表达显著增高(P<0.05),其中药物加手法组的改善效果更佳,手法组大鼠血清和乳腺组织中E_(2)、P的含量及ERα、PR的蛋白表达较模型组无明显差异(P>0.05)。结论循经通络推拿手法结合三苯氧胺对乳腺增生大鼠的治疗作用优于单纯手法和单纯药物治疗,其作用机制可能与调节大鼠体内雌孕激素及其受体水平相关。
Objective To observe the therapeutic effect of massage along meridians for dredging collaterals on mammary gland hyperplasia model(MGH)rats,and to compare the therapeutic effects of massage alone,drug therapy and manipulation combined with drug therapy,and to explore the mechanism of its therapeutic effect on regulating the sex hormone level in rats.Methods Fifty SD rats were randomly divided into 5 groups:a control group,a model group,a manipulation group,a drug group and a drug plus manipulation group,with ten rats in each group.Except for the control group,the other groups were injected with estradiol benzoate(0.5 mL/kg)for 25 days continuously,followed by progesterone(5 mg/kg)for 5 days to establish an MGH model.The manipulation group was treated with massage along meridians,the drug group was treated with tamoxifen(0.5 mg/kg)by gavage,and the drug plus manipulation group was treated with massage along meridians and medicine by gavage,all for 30 consecutive days.The general signs of rats in each group were observed.The diameter and height of the second pair of left and right nipples were measured.The pathological changes of mammary gland were observed by HE staining.The contents of estradiol(E_(2))and progesterone(P)in serum were detected by ELISA.The protein expressions of estrogen receptorα(ERα)and progesterone receptor(PR)in mammary gland were detected by Western-blot.Results Compared with the blank group,the height and diameter of nipples in the model group were significantly increased(P<0.05),the hyperplasia of breast tissue was obvious,the content of E_(2)in serum was significantly increased,the content of P was significantly decreased(P<0.05),the protein expression of ERαin breast tissue was significantly up-regulated,and the expression of PR was down-regulated(P<0.05).Compared with the model group,the diameter and height of nipples in the manipulation group,the drug group and the drug plus manipulation group were significantly reduced(P<0.05);the morphology of mammary gland tissue in the drug plus
作者
李丽
纪宁
阮迪
李丹丹
LI Li;JI Ning;RUAN Di;LI Dandan(Department of Massage,Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830099,China)
出处
《吉林中医药》
2023年第9期1078-1083,共6页
Jilin Journal of Chinese Medicine
基金
新疆维吾尔自治区自然科学基金(2018D01C287)。
关键词
循经通络推拿
乳腺增生
雌二醇
孕酮
雌激素受体
孕激素受体
大鼠
massage along meridians for dredging collaterals
hyperplasia of mammary glands
estradiol
progesterone
estrogen receptor
progesterone receptor
rats
