摘要
目的 研究甲胎蛋白阴性肝细胞癌(AFP-NHCC)患者血清外泌体环状RNA(circ RNA)100338,长链非编码RNA结直肠肿瘤差别表达(colorectal neoplasia differentially expressed,CRNDE)基因表达及临床预后价值。方法 选取自2018年1月~2019年7月期间联勤保障部队第九四二医院诊治的AFP-NHCC患者98例为研究对象,以同期健康体检的70例健康人作为对照组。实时荧光定量PCR检测血清外泌体circ RNA 100338和CRNDE水平。比较不同临床病理特征AFP-NHCC患者血清外泌体circ RNA 100338和CRNDE水平。受试者工作特征曲线(ROC)分析外泌体circ RNA 100338,CRNDE评估BCLC分期B~C期、低分化程度及肝内淋巴结转移的临界值。Kaplan-Meier生存分析(Log-Rank检验)分析血清外泌体circ RNA 100338,CRNDE对AFP-NHCC患者生存预后的影响。单因素及多因素COX回归分析影响AFP-NHCC患者预后的因素。结果 AFP-NHCC组患者血清外泌体circ RNA100338(2.78±0.67),CRNDE的相对表达量(3.05±0.70)高于对照组(0.92±0.31,1.06±0.29),差异具有统计学意义(t=21.619,22.435,均P <0.05)。AFP-NHCC组患者血清外泌体circ RNA100338与CRNDE表达呈显著正相关性(r=0.657,P <0.05)。BCLC分期B~C期(3.43±0.69,3.71±0.75)、低分化程度(3.64±0.73,4.27±0.74)及并发肝内淋巴结转移(3.96±0.79,4.39±0.76)AFP-NHCC患者血清外泌体circ RNA100338,CRNDE表达分别高于BCLC分期0~A期(1.87±0.60,2.13±0.61)、高中分化程度(2.02±0.64,1.97±0.61)及无肝内淋巴结转移(2.03±0.60,2.20±0.65)患者,差异均有统计学意义(t=11.648,11.707,13.699;11.100,16.857,15.211,均P <0.05)。外泌体circ RNA100338评估BCLC分期B~C期、低分化程度及肝内淋巴结转移的临界值分别为3.51,3.69和4.06,外泌体CRNDE评估BCLC分期B~C期、低分化程度及肝内淋巴结转移的临界值分别为3.79,4.31和4.45。circ RNA100338高表达组和低表达组患者三年总体生存率分别为52.08%(25/48)和86.00%(43/50)。circ RNA 100338高表达组患者累积生存�
Objective To study the expression of serum exosomes circ RNA(circ RNA)100338 and long chain non-coding RNA colorectal neoplasia differentially expressed(CRNDE)in alpha fetoprotein negative hepatocellular carcinoma(AFP-NHCC)and their clinical prognostic value.Methods 98 patients with AFP-NHCC diagnosed and treated in the Joint Service Support Force 942 Hospital from January 2018 to July 2019 were selected as the study object,and 70 healthy people in the same period were selected as the control group.The levels of circ RNA100338 and CRNDE in serum exosomes were detected by real-time fluorescent quantitative PCR,and the serum exocrine circ RNA100338 and CRNDE levels in AFP-NHCC patients with different clinical features were compared.The receiver operating curve(ROC)was used to analyze the cutoff value of exosomes circ RNA100338,CRNDE in evaluating the BCLC stage B to C,the degree of poor differentiation,and the critical value of intrahepatic lymph node metastasis.Kaplan Meier survival analysis(Log Rank test)was used to analyze the effect of serum exosome circ RNA100338 and CRNDE expression on the prognosis of AFP NHCC patients.Univariate and multivariate COX regression analysis was used to analyze factors affecting the prognosis of patients with AFP-NHCC.Results The relative expression of serum exosomes circ RNA100338 and CRNDE in patients with AFP-NHCC group(2.78±0.67,3.05±0.70)was significantly higher than that in the control group(0.92±0.31,1.06±0.29),and the differences were statistically significant(t=21.619,22.435,all P<0.05).There was a significant positive correlation between circ RNA100338 and CRNDE expression in serum exosomes of AFP-NHCC group(r=0.657,P<0.05).The expression of serum exosomes circ RNA100338 and CRNDE in AFP-NHCC patients with BCLC stage B~C(3.43±0.69,3.71±0.75),low differentiation(3.64±0.73,4.27±0.74)and intrahepatic lymph node metastasis(3.96±0.79,4.39±0.76)were significantly higher than those in patients with BCLC stage 0~A(1.87±0.60,2.13±0.61),high and middle differentiat
作者
段世举
DUAN Shiju(Department of Clinical Laboratory and Pathology,Joint Service Support Force 942 Hospital,Yinchuan 750001,China)
出处
《现代检验医学杂志》
CAS
2023年第5期63-69,共7页
Journal of Modern Laboratory Medicine
基金
宁夏回族自治区科技支撑计划项目(XT201817):多个血清指标联合对于甲胎蛋白阴性肝癌患者术后预后价值分析。
