摘要
该研究通过采用高效液相色谱(HPLC)法测定苯乳酸含量,从50株鱼肠道来源的乳酸菌中筛选高产苯乳酸的菌株,通过形态观察、生理生化试验及分子生物技术对其进行菌种鉴定,并以苯乳酸产量为响应值,通过单因素试验和响应面试验对其产苯乳酸发酵条件进行优化。结果表明,筛选到1株产苯乳酸能力较好的菌株DY2,其苯乳酸产量达2.45 mg/mL,经鉴定,菌株DY2为融合魏斯氏菌(Weissella confuse)。菌株DY2产苯乳酸的最适发酵条件为初始pH 6.0,发酵时间50 h,接种量3%、发酵温度34℃。在此优化条件下,苯乳酸产量达2.99 mg/mL,是优化前的1.22倍。
Phenyllactic acid content was determined by HPLC,and the strains with high-yield of phenyllactic acid were screened from 50 strains of lactic acid bacteria(LAB)derived from fish intestines.The strains were identified by morphological observation,physiological and biochemical tests and molecular biology techniques.Taking the yield of phenyllactic acid as the response value,the fermentation conditions of phenyllactic acid production were optimized by single factor tests and response surface tests.The results showed that a strain DY2 with better phenyllactic acid production ability was screened,and the yield of phenyllactic acid reached 2.45 mg/ml.The strain DY2 was identified as Weissella confuse.The optimal fermentation conditions for phenyllactic acid production by strain DY2 were as follows:initial pH 6.0,fermentation time 50 h,inoculum 3%,and temperature 34℃.Under these optimized conditions,the yield of phenyllactic acid reached 2.99 mg/ml,which was 1.22 times that of before optimization.
作者
吕孟敏
白凤翎
崔方超
檀茜倩
吕欣然
励建荣
郭晓华
LV Mengmin;BAI Fengling;CUI Fangchao;TAN Xiqian;LV Xinran;LI Jianrong;GUO Xiaohua(National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,The Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities,College of Food Science and Technology,Bohai University,Jinzhou 121013,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,China;Shandong Meijia Group Co.,Ltd.,Rizhao 276800,China)
出处
《中国酿造》
CAS
北大核心
2023年第9期125-130,共6页
China Brewing
基金
“十三五”国家重点研发计划项目课题(2019YFD0901702)
辽宁省锦州市科技指导性计划项目(JZ2022B031)。
关键词
苯乳酸
乳酸菌
鱼肠道
筛选
鉴定
发酵条件
响应面优化
phenyllactic acid
lactic acid bacteria
fish intestine
screening
identification
fermentation condition
response surface optimization
