摘要
目的采用药用阳离子交换树脂(聚磺苯乙烯钠)在体外进行中药汤剂“肾衰方”中K^(+)的去除研究,建立中药汤剂的体外去除K^(+)工艺。方法采用电感耦合等离子体质谱法测定汤剂中的K^(+)和Na^(+)浓度,采用超高效液相色谱测定汤剂中各指标成分含量的变化。经树脂性能的考察,确定采用阳离子树脂静态吸附法,并初步判定K^(+)去除的影响因素有温度、树脂用量、吸附时间。采用单因素试验研究这3个因素对水煎液中K^(+)吸附的影响。结果最佳工艺条件:温度为25℃,树脂用量为汤剂∶树脂(15 mL∶1 g),吸附时间为1 min。本工艺应用于中药“肾衰方”K^(+)的去除率>80%,应用于临床不同肾病处方的K^(+)去除率为62.5%~80.3%,极大地降低了肾衰患者服用中药汤剂K^(+)的摄入。研究发现此药用树脂对K^(+)的吸附作用受离子环境的影响较大,对各指标成分的吸附也具有一定的选择性。结论建立的去钾工艺能够很好地去除“肾衰方”汤剂中的K^(+)。
OBJECTIVE To conduct the removal of K^(+)in traditional Chinese medicine decoction of Shenshuai Recipe by using the medicinal cation exchange resin(sodium polystyrene sulfonate).METHODS The concentration of K^(+)and Na^(+)in the decoction were determined by the ICP-MS method.And the UPLC method was established to determined the impact of K^(+)removal technique on indicative compounds in the decoction.After investigating the resin properties,static adsorption of cation exchange resin was applied in the study.It was preliminarily determined that the factors affecting K^(+)removal was temperature,resin amount and adsorption time.The single factor experiment was conducted to investigated the influence of these three factors on K^(+)adsorption in decoction.RESULTS The appropriate process conditions were determined as follows:the temperature was 25℃,the amount of resin was decoction︰resin(15 mL︰1 g),and the adsorption time was 1 min.The removal rate of K^(+)was>80%in decoction of Shenshuai Recipe.The removal rate of K^(+)was 62.5%−80.3%in other decoction of renal disease prescription.This technology was greatly reduced the intake of K^(+)through decoction in patients with renal failure.In addition,it was found that the adsorption effect of this medicinal resin on K^(+)was greatly affected by the ion environment,and this kind of resin was also selective for the adsorption of different indicative component.CONCLUSION The establishment of this process of K^(+)removal can well reduce K^(+)in the decoction of Shenshuai Recipe.
作者
王芳宇
孙浩
徐英
梁颖
张慧杰
WANG Fangyu;SUN Hao;XU Ying;LIANG Ying;ZHANG Huijie(Hospital Affiliated to Tianjin Academy of Traditional Chinese Medicine,Department of Pharmacy,Tianjin 300120,China;Hospital Affiliated to Tianjin Academy of Traditional Chinese Medicine,Chinese Medicine Research Institute,Tianjin 300120,China;Hospital Affiliated to Tianjin Academy of Traditional Chinese Medicine,Department of Nephrology,Tianjin 300120,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2023年第16期2243-2250,共8页
Chinese Journal of Modern Applied Pharmacy
基金
天津市卫生健康委员会、天津市中医药管理局中医中西医结合科研课题(2019025,2021182)
天津市中医药研究院附属医院院内培育基金项目(2020003)。
关键词
肾衰方
钾离子
阳离子交换树脂
聚磺苯乙烯钠
静态吸附
Shenshuai Recipe
potassium ion
cation exchange resin
sodium polystyrene sulfonate
static adsorption
