摘要
目的基于16S rRNA基因测序技术分析肺脾两虚型慢性阻塞性肺疾病(COPD)大鼠模型肠道菌群的特点。方法Wistar大鼠30只,随机分为空白对照组、模型组,每组各15只。模型组以烟熏法+冰冷番泻叶浸液灌胃法建立肺脾两虚型COPD模型,造模后收集两组大鼠粪便,行肠道菌群16S rRNA测序,分析肠道菌群结构、多样性和丰度变化。结果基于操作分类单位(OTU)的韦恩图结果显示,模型组较空白对照组的OTU数量下降;菌群结构分析显示,空白对照组以厚壁菌门为主,模型组以拟杆菌门为主;属水平上空白对照组以厚壁菌门中的乳杆菌数量最多,模型组拟杆菌数量最多,乳杆菌数量较空白对照组下降。主坐标分析(PCoA)分析表明两组在菌群组成上存在差异,在门水平上,与空白对照组比较,模型组放线菌门下降,变形菌门和脱硫菌门上升;在属水平上,与空白对照组比较,模型组拟杆菌属、罗姆布茨菌属、毛螺杆菌属及副拟杆菌属上升,普雷沃氏菌属、梭菌属、布氏菌属和瘤胃球菌下降。菌群扩增子α多样性分析显示,模型组香农(shannon)指数和丰富度实际观测值(sobs)指数均低于空白对照组。结论肺脾两虚型COPD模型大鼠较正常大鼠发生了菌群结构和相对丰度、菌群多样性的改变。
OBJECTIVE To analyze the characteristics of intestinal flora in rats with chronic obstructive pulmonary disease(COPD)of lung and spleen deficiency based on 16SrRNA gene sequencing technology.METHODS Totally 30Wistar rats were randomly divided into the blank control group and the model group,with 15rats in each group.The rats of the model group were treated with fumigation plus cold senna leaf infusion to establish COPD models of lung and spleen deficiency.The stool samples were collected from the two groups after the modeling.16SrRNA sequencing was carried out for the intestinal flora,and the changes of structure,diversity and abundance of intestinal flora were observed.RESULTS The result of Wayne diagram based on operational taxonomic unit(OTU)showed the number of OUT of the model group was less than that of the blank control group.The analysis of structure of flora showed that Firmicutes was dominant in the blank control group,and Bacteroides was dominant in the model group.At the genus level,Lactobacillus was dominant among Firmicutes in the blank control group,and Bacteroides was dominant in the model group,and the counts of Lactobacillus of the model group were less than those of the blank control group.Principal coordinate analysis(PCoA)indicated that there was significant difference in the composition of intestinal flora between the two groups;at the phyla level,the Actinomycetes counts of the model group were less than those of the blank control group,while the Proteus and Desulfobacter counts of the model group were more than those of the blank control group.At the genus level,the Bacteroides,Rombucella,Trichospirillum and Parabacteroide counts of the model group were more than those of the blank control group,while the Prevotella,Clostridium,Brucella and Rumen coccus counts of the model group were less than those of the blank control group.Diversity of flora showed that Shannon index and sobs index of the model group were lower than those of the control group.CONCLUSION The structure,relative abundance
作者
胡旭贞
洪波
陈希尔
HU Xu-zhen;HONG Bo;CHEN Xi-er(Ningbo Municipal Hospital of TCM,Ningbo,Zhejiang315000,China)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2023年第14期2086-2090,共5页
Chinese Journal of Nosocomiology
基金
浙江省宁波市科技计划基金资助项目(2019A610365)。
关键词
慢性阻塞性肺疾病
肺脾两虚
肠道菌群
16S
rRNA基因测序技术
大鼠模型
Chronic obstructive pulmonary disease
Lung and spleen deficiency
Intestinal flora
16SrRNA gene sequencing technology
Rat model
