摘要
目的:研究迷迭香酸(RA)对人食管鳞癌EC9706细胞增殖的影响和潜在作用机制.方法:通过CCK8实验和葡萄糖消耗实验评估RA对EC9706细胞增殖和糖代谢的影响;用微小RNA(miRNA)测序筛选在RA作用下差异性表达miRNA;荧光定量qPCR和Western blot检测miR-223-3p、缺氧诱导因子(HIF)-1α和葡萄糖转运蛋白(GLUT)4的差异化表达;检测转染miR-223-3p合并RA作用下,EC9706细胞葡萄糖消耗、HIF-1α和GLUT4表达的变化.结果:在RA作用下,EC9706细胞增殖、葡萄糖消耗量、miR-223-3p和HIF-1α表达均显著下降,但对GLUT4表达无影响;在miR-223-3p过表达情况下,RA下调EC9706细胞的葡萄糖消耗量和HIF-1α表达,但对GLUT4表达没影响.结论:RA抑制人食管鳞癌EC9706细胞增殖,并下调其葡萄糖代谢能力和miR-223-3p表达,但作用机制并不通过miR-223-3p/GLUT4轴.
Objective:To investigate the effect of Rosmarinic acid(RA)on the proliferation of Esophageal carcinoma EC9706 cells and the potential mechanism.Methods:CCK8 assay and glucose depletion assay were performed to assess the effects of RA on EC9706 cell proliferation and glucose metabolism;microRNA(miRNA)sequencing was applied to screen differentially expressed miRNA associated with glucose metabolic pathway under RA treatment;qPCR and Western blot were used to detect hsa-miR-223-3p,hypoxia inducible factor(HIF)-lαand recombinant glucose transporter(GLUT)4 expression;Glucose depletion,HIF-1αand GLUT4 expression were detected in EC9706 cells under miR-223-3p transfection combining RA treatment.Results:The proliferation,glucose consumption,miR-223-3p and HIF-1αexpression of EC9706 cells were significantly decreased under RA,but Glut4 expression was not affected.The same phenomena were detected under miR-223-3p over-expression combining RA treatment.Conclusion:Rosmarinic acid inhibited the proliferation of esophageal carcinoma EC9706 cells and down-regulated glucose metabolism and miR-223-3p expression,but the mechanism was not through the miR-223-3p/CLUT4 axis.
作者
林清凡
温扬敏
罗彩林
LIN Qing-fan;WEN Yang-min;LUO Cai-lin(Quanzhou Medical College,Quanzhou 362011,China)
出处
《四川解剖学杂志》
2023年第2期1-5,共5页
Sichuan Journal of Anatomy
基金
福建省泉州市科技计划项目(2018N107S)
福建省教育厅中青年教师教育科研项目(JAT171165)资助。
