摘要
为探明湘黔山区稻田养殖呆鲤群体的遗传多样性现状,采用14对微卫星引物对湘西永顺、怀化藕团、黔东南锦屏县的呆鲤养殖群体与湘江野鲤自然群体、兴国红鲤养殖群体的遗传多样性及群体间的遗传分化水平进行研究。结果显示:14个微卫星位点平均等位基因数为16.571~19.214,平均有效等位基因数为9.420~11.143,各群体的平均遗传观测杂合度为0.705~0.778,平均期望杂合度为0.884~0.893,平均多态信息含量为0.883~0.891;各位点群体间的遗传分化系数平均值为0.017,基因流为3.088~27.730,5%的遗传变异来自于群体间,而95%的变异来源于群体内个体间和个体内。试验结果表明,本次采集的5个群体均具有较高的遗传多样性,群体间存在一定的基因流动且遗传分化较弱,在长期的稻田养殖条件下,呆鲤群体遗传多样性未下降,未与湘江野鲤产生明显分化,但藕团呆鲤与湘江野鲤产生了一定的遗传分化。
In order to investigate the genetic information on the staycarp adapted to paddy field conditions in Hunan and Guizhou, fourteen microsatellites were used to evaluate the genetic diversity and genetic differentiation of three “Stay carp” populations from Yongshun Xiangxi, Outuan Huaihua, Jinping Qiandongnan, one red carp population from Fisheries Research Institute of Hunan Province, one wild Cyprinus carpio population from Xiangjiang. The results showed that 14 pairs of microsatellite primers were amplified with clear bands 16.571—19.214 average numbers of alleles, and 9.420—11.143 effective alleles per pair. The average observed heterozygosity was 0.705 to 0.778, while mean expected heterozygosity ranged from 0.884 to 0.893, the value of average polymorphism information content was 0.883 to 0.891. The genetic differentiation index among the 5 populations, the gene flow values all locihe genetic variation among populations, variation within populations. These findings that the 5 carp populations hd high level of genetic diversity, of gene flow the 5 populations, and genetic differentiation. The “stay carp” population still kept high genetic diversity, and genetic differentiation with wild population from Xiangjiang.
作者
邹利
王金龙
李传武
王冬武
曾春芳
刘明求
刘丽
谢敏
曾鸣
ZOU Li;WANG Jinlong;LI Chuanwu;WANG Dongwu;ZENG Chunfang;LIU Mingqiu;LIU Li;XIE Min;ZENG Ming(Fisheries Research Institute of Hunan Province,Changsha 410153,China;Changde Research Center for Agricultural Biomacromolecule,Zoology Key Laboratory of Hunan Higher Education,Hunan Provincial Key Laboratory for Health Aquaculture and Product Processing in Dongting Lake Area,Hunan University of Arts and Science,Changde 415000,China)
出处
《水产科学》
CAS
CSCD
北大核心
2023年第5期795-804,共10页
Fisheries Science
基金
国家重点研发计划项目(2020YFD0900302)
湖南省现代农业产业技术体系项目(湘农发[2019]26号)
湖南文理学院博士启动基金资助项目(21BSQD01)。
关键词
呆鲤
微卫星
遗传多样性
遗传分化
stay carp
microsatellite
genetic variability
genetic differentiation
