摘要
为明确苹婆Sterculia monosperma炭疽病的病原菌暹罗炭疽菌Colletotrichum siamense的生物学特性,筛选有效的杀菌剂,用生长速率法测定了苹婆炭疽病病原菌最适生长温度、pH值、碳源、氮源等生物学特性,并测定了6种杀菌剂对其的室内毒力。研究结果表明,暹罗炭疽菌最适碳源为纤维素粉和乳糖,最适氮源为胰蛋白胨,最适pH值为7.0,最佳生长温度范围为28~30℃,致死温度为55℃,不同光照条件下对其生长影响差异不显著。室内毒力测定结果表明98%溴菌腈、97%嘧霉胺、95%啶酰菌胺、98%嘧菌酯、96.8%苯醚甲环唑和98%福美双对病原菌菌丝生长均有抑制效果,其中96.8%苯醚甲环唑的抑制效果最佳,EC_(50)值为5.24μg/mL。
To identify the biological characteristics of Colletotrichum siamense,a leaf pathogen of Sterculia monosperma,and to screen effective fungicides,the biological characteristics of C.siamense,such as the light exposure,pH,carbon source,nitrogen source and temperature,were determined by the method of growth rate.The 6 fungicides displayed significant but varying inhibitory effects on mycelial development.The results showed that cellulose and lactose was the most suitable carbon source,tryptone was the preferred nitrogen source.And C.siamense grew well on PDA medium at the pH of 7.0 and 28-30°C,the lethal temperature was 55°C.Different light exposures did not significantly affect the fungal growth.The results of the indoor toxicity test showed that 98%bromothalonil,97%pyrimethanil,95%boscalid,98%azoxystrobin,96.8%difenoconazole and 98%thiram had inhibitory effects on the mycelial growth of pathogens.For those fungicides,96.8%difenoconazole showed high toxicity with EC_(50) of 5.24μg/mL.
作者
王成
赵丹阳
扈丽丽
赵奕
高成龙
张心结
陈诗琦
黄华毅
WANG Cheng;ZHAO Danyang;HU Lili;ZHAO Yi;GAO Chenglong;ZHANG Xinjie;CHEN Shiqi;HUANG Huayi(Longyandong Forest Farm of Guangdong Province,Guangzhou,Guangdong 510520,China;Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization/Guangdong Academy of Forestry,Guangzhou,Guangdong 510520,China;College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,Guangdong 510642,China)
出处
《林业与环境科学》
2023年第4期21-26,共6页
Forestry and Environmental Science
基金
广东省林业科技创新项目(2020KJCX004、2023KJCX022)。
关键词
苹婆
暹罗炭疽菌
生物学特性
毒力测定
Sterculia monosperma
Colletotrichum siamense
biological characteristics
toxicity tests
