摘要
目的探讨双淬灭荧光探针在肠癌患者肠道菌群荧光定量(qPCR)检测中的应用效果。方法选取2020年1月至2022年8月接受手术的42例肠癌患者为研究组,选取健康体检成人38例为对照组,收集手术前后益生菌制剂干预4周的粪便标本,采用常规qPCR和双淬荧光探针qPCR检测肠道菌群的表达量。结果研究组的胆汁酸、血清总胆汁酸、次级胆汁酸均高于对照组(P<0.05)。两组患者手术前后的大肠杆菌、葡萄球菌、变形杆菌、双歧杆菌、嗜酸乳杆菌比较有统计学差异(P<0.05)。结论双淬灭荧光探针检测肠道菌群、胆汁酸的方法便捷,结果准确,对鉴别及筛查早期肠癌患者有指导意义,值得临床推广。
Objective To explore the application effect of double quenched fluorescent probe in the detection of intestinal flora quantitative fluorescence(qPCR)in patients with colorectal cancer.Methods 42 patients with colorectal cancer undergoing surgery from January 2020 to August 2022 were selected as study group,and 38 healthy adults were selected as control group.Fecal samples were collected before and after 4 weeks of intervention with probiotics.The expression levels of intestinal flora were detected by routine qPCR and double quenched fluorescence probe qPCR.Results The bile acids,serum total bile acids and secondary bile acids of the study group were higher than those of the control group(P<0.05).Statistical difference was found in the Escherichia coli,Staphylococcus,Proteus mirabilis,Bifidobacteria,and Lactobacillus acidophilus before and after surgery between the two groups(P<0.05).Conclusions Double quenched fluorescent probe in the detection of intestinal flora and bile acid is convenient and accurate,which is of guiding significance for the identification and screening of patients with early colorectal cancer,and is worthy of clinical promotion.
作者
臧彩霞
奚晓华
朱会芳
陈丹丹
臧丽
ZANG Caixia;XI Xiaohua;ZHU Huifang;CHEB Dandan;ZANG Li(Clinical Laboratory,Fuliang County People's Hospital,Jingdezhen 333400,China)
出处
《临床医学工程》
2023年第9期1199-1200,共2页
Clinical Medicine & Engineering
基金
江西省卫生健康委科技计划项目(202212044)。
关键词
双淬灭荧光探针
肠癌
肠道菌群
荧光定量PCR
Double quenched fluorescent probe
Colorectal cancer
Intestinal flora
Fluorescence quantitative PCR
