摘要
以水面无土栽培水芹品种金陵1号为材料,通过液相色谱串联电喷雾质谱法(LC-SIM-Orbitraq)测定不同器官中的有机酸种类和含量。经过色谱条件的优化,25种有机酸得到较好的分离,在1×10^(-3)~10μg·mL^(-1)的质量浓度范围内线性关系良好,相关系数在0.9910~0.9999之间;在0.5、5、20μg·mL^(-1)的添加质量浓度下,加标回收率在50.39%~125.32%之间;样品稳定性较好,日内稳定性在0.28%~3.99%之间,日间稳定性在0.41%~6.63%之间。结果表明,金陵1号水芹不同器官中有机酸以草酸、苹果酸、琥珀酸、柠檬酸、奎尼酸和莽草酸为主,其中,根中草酸含量(w)最高,达(32094.08±1600.77)μg·g^(-1),这可能是水芹环境适应性强的主要原因之一;而茎、叶中苹果酸、琥珀酸和柠檬酸含量较高,说明茎、叶口感风味更佳;此外,奎尼酸和莽草酸在茎、叶中含量相对较高,可将水芹用于奎尼酸和莽草酸的提取或功能保健食品的开发,具有较大的资源化利用潜力。
The species and content of organic acids in different organs of soilless cultivated water dropwort jinling No.1 were determined by liquid chromatography single ion monitor orbitraq(LC-SIM-Orbitraq).After the optimization of chromatographic conditions,25 organic acids were well separated,and the linear relationship was good within the mass concentration range of 1×10^(-3)^(-1)0μg·mL^(-1),and the correlation coefficient was between 0.9910 and 0.9999.At the added concentration of 0.5,5,and 20μg·mL^(-1),the recovery rates of add standard were in the range of 50.39%-125.32%.The samples have a superior stability,the intra-day stability ranged from 0.28%to 3.99%and the inter day stability ranged from 0.41%to 6.63%.The results show that the major parts of organic acids in water dropwort jinling No.1 are oxalic acid,succinic acid,malic acid,shikimic acid,citric acid and D-(-)-quinic acid.The oxalic acid in the root was the highest,reaching(32094.08±1600.77)μg·g^(-1),which may be one of the main reasons for the strong environmental adaptability of water dropwort.The proportions of malic acid,succinic acid and citric acid in stems and leaves were higher than roots,indicating that stems and leaves had better taste and flavor.Moreover,the contents of D-(-)-quinic acid and shikimic acid in stems and leaves is higher than roots.Therefore,water dropwort can be used for D-(-)-quinic acid and shikimic acid extraction or functional health food development,and has great potential for resource utilization.
作者
刘杰
刘吉祥
常雅军
陈婷
刘晓静
孙林鹤
姚东瑞
LIU Jie;LIU Jixiang;CHANG Yajun;CHEN Ting;LIU Xiaojing;SUN Linhe;YAO Dongrui(Nanjing Wondux Environmental Protection Technology Co.,Ltd.,Nanjing 211122,Jiangsu,China;Jiangsu Engineering Research Center of Aquatic Plant Resources and Water Environment Remediation/Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-sen),Nanjing 210014,Jiangsu,China)
出处
《中国瓜菜》
CAS
北大核心
2023年第9期80-86,共7页
China Cucurbits And Vegetables
基金
江苏省农业科技自主创新资金项目〔CX(21)2012〕
江苏省苏北科技专项(HAQFM202004)
江苏省水利科技项目(2021051)
江苏省六大人才高峰高层次创新人才团队项目(TD-JNHB-008)。
