摘要
目的:探讨结直肠上皮细胞活性氧(ROS)及亚铁原卟啉(FH)物质检测对于结直肠癌筛查的应用价值。方法:选取南京大学医学院附属鼓楼医院消化内镜中心2021年9月至2022年1月进行结肠镜检查的患者220例,所有患者均行结肠镜组织学病理活检及ROS+FH试剂盒检测,分别计算其灵敏度、特异度、约登指数等各评价指标,并进行Kappa检验,以此探究ROS及FH对于结直肠癌筛查的价值。结果:在220例患者病理结果中,结直肠癌患者样本20例,非结直肠癌患者样本200例。在结直肠癌患者中,ROS及FH检测的灵敏度分别为95%和100%;特异度分别为99%和69%;Kappa系数分别为0.919和0.288(P均<0.001)。结论:结直肠上皮细胞ROS及FH对于诊断均有较高的灵敏度、特异度,其中ROS检测结果与结直肠癌病理结果有极高的一致性,可作为临床早期结直肠癌筛查的简便指标。
Objective To determine the utility of cell free ferrous protoporphyrin(FH)substance detection and colorectal epithelial cell reactive oxygen species(ROS)for colorectal cancer screening.Methods At the Gastrointestinal Endoscopy Center of Nanjing Drum Tower Hospital,The Affiliated Hospital of Nanjing University Medical School,220 patients underwent colonoscopies between September 2021 and January 2022.Each patient also received colonoscopic histopathological biopsy,ROS,and FH kits.To determine the efficacy of ROS and FH for colorectal cancer screening,the Kappa test was used.Results Among the 220 patients with pathological results,twenty samples were from colorectal cancer patients and 200 samples were from non-colorectal cancer patients.In colorectal cancer patients,the sensitivities of ROS and FH assays were 95%and 100%,respectively;the specificities were 99%and 69%,respectively;and the Kappa values were 0.919 and 0.288,respectively(both P<0.001).Conclusions The results of ROS detection are in high agreement with the pathological results of colorectal cancer,and the ROS and FH of colorectal epithelial cells have high sensitivity and specificity.
作者
孙文琦
吴欣荣
王运荣
赵贝
窦晓坛
李雯
邹晓平
王雷
陈敏
Wenqi Sun;Xinrong Wu;Yunrong Wang;Bei Zhao;Xiaotan Dou;Wen Li;Xiaoping Zou;Lei Wang;Min Chen()(Department of Gastroenterology,the Affiliated Drum Tower Hospital of Nanjing University,Medical School,Nanjing 210008,China)
出处
《中华结直肠疾病电子杂志》
2023年第4期326-330,共5页
Chinese Journal of Colorectal Diseases(Electronic Edition)
关键词
结直肠肿瘤
结直肠上皮细胞
活性氧
细胞游离亚铁原卟啉
早期筛查
Colorectal neoplasms
Colorectal epithelial cell
Reactive oxygen species
Cell free ferrous protoporphyrin
Early screening
