摘要
以中国野生‘燕山’葡萄(Vitis yeshanensis‘Yanshan’)为试材,克隆VyCIPK3基因,GenBank登录号为NM_001281283,利用实时定量PCR(qPCR)技术分析了VyCIPK3基因在葡萄不同组织器官中,以及不同植物生长调节剂、非生物胁迫诱导下葡萄中的基因表达,以期为进一步研究葡萄CIPK基因的分子功能及探讨葡萄抗逆分子机制提供参考依据。结果表明:VyCIPK3基因表达具有组织特异性,在根中高表达;VyCIPK3基因对脱落酸(ABA)和茉莉酸甲酯(MeJA)诱导响应强烈,VyCIPK3基因在不同逆境胁迫中,对盐胁迫响应更为强烈。VyCIPK3基因在‘燕山’葡萄生长发育和抵御外界逆境胁迫方面起着重要的作用,该研究有助于了解中国野生葡萄的抗逆机制,为葡萄抗逆育种提供基因资源。
Taking Chinese wild‘Yanshan’grape(Vitis yeshanensis‘Yanshan’)as the material,we cloned the VyCIPK3 gene,with GenBank accession number NM_001281283,and analyzed the gene expression of the VyCIPK3 gene in different tissues and organs of grapes,as well as in grapes under the induced by different plant growth regulators and abiotic stresses by using quantitative realtime PCR(qPCR),in order to provide reference for the further study on the molecular function of the CIPK gene in grapes and the exploration of molecular mechanisms of grapevine stress resistance.The results showed thatVyCIPK3gene expression was tissue specific and highly expressed in roots.TheVyCIPK3gene responded strongly to abscisic acid(ABA)and methyl jasmonate(MeJA)induction,and theVyCIPK3gene responded more strongly to salt stress among different adversity stresses.TheVyCIPK3gene played an important role in‘Yanshan’grape growth and development and protection against external adversity stress,and this study might help to understand the mechanism of stress resistance in wild grapes in China and provide a gene resource for grape stress resistance breeding.
作者
刘砚璞
肖雪
赵建明
朱自果
LIU Yanpu;XIAO Xue;ZHAO Jianming;ZHU Ziguo(School of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang,Henan 453003;Henan Research Center for Horticultural Plant Resources Utilization and Germplasm Innovation Engineering,Xinxiang,Henan 453003;Shandong Grape Research Institute,Jinan,Shandong 250000)
出处
《北方园艺》
CAS
北大核心
2023年第15期33-40,共8页
Northern Horticulture
基金
山东省重大科技创新工程资助项目(2019JZZY010727)。
