摘要
为了获取杉叶的化学成分,以广西种植的杉木杉叶为材料,采用液相色谱-质谱联用(LC-MS)技术采集杉叶中化学成分的一、二级质谱数据,将质谱数据在全球天然产物社会分子网络(GNPS)平台上进行数据转换,根据碎片离子的相似性建立簇,得到分子网络。结合色谱保留时间、精确分子质量、特征碎片离子裂解规律以及文献报道等,分别鉴定各化合物的化学结构。结果表明:①根据液相色谱-质谱联用(LC-MS)技术对杉叶的检测及分子网络分析结果,识别出双黄酮类、原花青素类、黄酮类、黄酮苷类、二萜类等5种类型分子簇;②在杉叶样品中共鉴定和推测出88个化合物,如穗花杉双黄酮、原花青素B1、金圣草黄素、槲皮苷、美丽红豆杉素G等成分;③推测分析出杉叶中含有2个潜在的新化合物,均为萜类化合物。
In order to get the chemical components of the leaves of Cunninghamia lanceolata in Guangxi were used as materials,the primary and secondary mass spectrometry data were collected by liquid chromatography mass spectrometry(LC-MS),the MS/MS data of the C.lanceolata leaves was converted on the platform of Global Natural Products Social Molecular Networking(GNPS),and clusters were established according to the similarity of fragment irons,thus obtaining a molecular network.According to the comparison of chromategraphic retention time,accurate molecular mass,characteristic fragment ions,MS/MS cracking rules and literatures,the compounds were identified.The results showed:(1)Five types of particle clusters were identified in the molecular network,including biflavones,proanthocyanidins,flavonoids,flavonoids glycosides and diterpenoids;(2)A total of 88 compounds were identified from C.lanceolata leaves,including amentoflavone,procyanidins B1,genistein,quercitrin,and Taxamairin G.(3)Two potential new compounds were speculative analyzed,which were terpene compounds.
作者
陆国寿
黄周锋
张赟赟
李冬梅
苏铁
高美美
閤雪晴
Lu Guoshou;Huang Zhoufeng;Zhang Yunyun;Li Dongmei;Su Tie;Gao Meimei;Xia Xueqing(Guangxi Institute of Traditional Medical and Pharmaceutical Sciences,Nanning 530022,P.R.China;Guangxi Key Laboratory of Traditional Chinese Medicine Quality Stardands;Guangxi Gaofeng Tree Farm;Guangxi University of Traditional Chinese Medicine)
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2023年第10期47-53,61,共8页
Journal of Northeast Forestry University
基金
广西中医药适宜技术开发与推广项目(GZSY21-04)
广西科技计划项目(桂科AD22035055)。
关键词
杉叶
液-质联用法
分子网络
化学成分
Leaves of Cunninghamia lanceolata
Liquid chromatography mass spectrometry(LC-MS)
Molecular networking
Chemical composition
