摘要
目的:观察参芪地黄汤化裁方对腺嘌呤慢性肾衰大鼠血清肌酐(SCr)、尿素氮(BUN)及肾脏病理改变的影响。方法:取Wistar大鼠60只,随机分为6组,分别为正常组、模型组、阳性组和参芪地黄汤化裁方低、中、高剂量组,每组10只;以腺嘌呤造模,检测各组大鼠血清SCr、BUN含量,进行组间比较分析。采用HE染色、Masson染色观察肾脏病理改变。结果:与正常组比较,模型组血清SCr、BUN含量显著升高(P<0.01);与模型组比较,阳性组、参芪地黄汤化裁方3剂量组SCr、BUN含量均有显著下降(P<0.05、P<0.01);HE染色显示模型组肾小球数量减少,囊腔扩张,肾小管扩张,管腔内可见黄色沉积物;Masson染色显示肾组织纤维化,结构显色区分不清。各给药组上述病理表现较模型组轻,其中阳性组和高剂量组病理改变减轻更明显。结论:参芪地黄汤化裁方能显著延缓腺嘌呤致慢性肾衰大鼠肾功的下降,并改善其肾组织病理变化。
Objective:To observe the effects of Modified Shenqi Dihuang Decoction on serum creatinine(SCr),urea nitrogen(BUN),and renal pathological changes in rats with chronic renal failure caused by adenine.Methods:60 Wistar rats were randomly divided into 6 groups:normal group,model group,positive drug group,and low,medium,and high dose groups of Modified Shenqi Dihuang Decoction,with 10 rats in each group;Using adenine as a model,the levels of SCr and BUN in each group of rats were measured for comparative analysis between groups.HE staining and Masson staining were used to observe renal pathological changes.Results:Compared with the normal group,the serum levels of SCr and BUN in the model group were significantly increased(P<0.01);Compared with the model group,there were significant differences(P<0.05,P<0.01)in the content of SCr and BUN between the positive drug group and the three dose groups with Modified Shenqi Dihuang Decoction;HE staining showed a decrease in the number of glomeruli,dilation of the capsule and renal tubules,and visible yellow deposits in the lumen in the model group;Masson staining showed renal ti-ssue fibrosis,with unclear structural color differentiation.The above pathological manifestations in each medication group were lighter than those in the model group,with the positive group and high-dose group showing more significant reduction in pathological changes.Conclusion:Modified Shenqi Dihuang Decoction can significantly delay the decline of renal function in rats with chronic renal failure caused by adenine,and improve the pathological changes of renal tissue.
作者
严浩军
徐晨希
刘芙蓉
张树明
YAN Haojun;XU Chenxi;LIU Furong;ZHANG Shuming(Heilongjiang Academy of Chinese Medicine Sciences,Harbin,Heilongjiang 150036)
出处
《中国中医药科技》
CAS
2023年第4期646-649,共4页
Chinese Journal of Traditional Medical Science and Technology
关键词
慢性肾衰
参芪地黄汤化裁方
肌酐
尿素氮
病理学
大鼠
chronic renal failure
Modified Shen qi Dihuang Decoction
creatinine
urea nitrogen
pathology
rat
