摘要
目的 基于真实世界数据挖掘与网络药理学探究中药外治口腔溃疡的用药规律及核心药对的作用机制。方法 采用中医传承辅助平台(V2.5)对药物频次和组方规律进行分析,挖掘筛选出高频药对。并采用网络药理学对药对活性成分、靶点进行分析和信号通路富集分析。结果 真实世界数据挖掘后筛选得到高频药对吴茱萸-冰片,网络药理学研究得到吴茱萸30种、冰片4种潜在有效成分,共569个靶点,确定174个交集靶点,与口腔溃疡炎症疾病的交点有40个,包括TNF、IL6、IL1-β、PTGS2等7个核心靶点,通过作用于核心靶点来调控相关信号通路(AGE-RAGE、IL-17、TNF等),发挥抗炎作用来治疗口腔溃疡。结论 通过真实世界数据挖掘初步确定吴茱萸-冰片核心药对,并基于网络药理学分析其多成分、多靶点、多通路的作用机制,为口腔溃疡真实世界的外用中药新药开发及临床治疗提供了科学依据。
Objective To investigate the drug use pattern and the mechanism of action of core drug pairs in the external treatment of oral ulcers in Chinese medicine based on real-world data mining and network pharmacology.Methods The frequency of drugs and the rule of prescription composition were analyzed by using the TCM inheritance assistance platform(V2.5),and high-frequency drug pairs were selected.Network pharmacology was used to analyze the active ingredients,target and signal pathway enrichment.Results The high-frequency drug pair Wuzhuyu-Bingpian was screened after real-world data mining,and the network pharmacology study yielded 30 potential active ingredients of Wuzhuyu and 4 potential active ingredients of Bingpian,with a total of 569 targets and 174 intersecting targets.There are 40 intersecting targets of drug pairs with inflammatory diseases of oral ulcers,including 7 core targets such as TNF,IL6,IL1-β,PTGS2,etc.By acting on the core targets to modulate relevant signaling pathways(AGE-RAGE,IL-17,TNF,etc.) to exert anti-inflammatory effects to combat mouth ulcers.Conclusion The core drug pair of Wuzhuyu-Bingpian was initially identified through real-world data mining,and its multi-component,multi-target and multi-pathway mechanism of action was analyzed based on network pharmacology,which provides scientific basis for the development of new topical herbal drugs for real-world oral ulcer and clinical treatment.
作者
陈杨春华
王静
袁子民
CHEN Yang-Chunhua;WANG Jing;YUAN Zi-Min(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)
出处
《中国药物经济学》
2023年第7期46-52,56,共8页
China Journal of Pharmaceutical Economics
基金
辽宁省自然科学基金资助项目(2019-ZD-0440)
辽宁省教育厅2019、2022年度高等学校基本科研项目(LJKZ0882、LJKMZ20221320)。
