摘要
建立定量核磁共振波谱法测定枣果实中葡萄糖、果糖、蔗糖3种可溶性糖含量的方法。样品用氘代水超声提取,以邻苯二甲酸氢钾为内标,Zgpr作为测定序列进行氢谱测定。结果表明,葡萄糖、果糖、蔗糖线性方程斜率的测定值与理论值误差小于2%,线性相关系数均大于0.9999,精密度RSD小于0.5%,3种糖的检出限分别为23.9、20.0、18.0μg/mL,定量限分别为79.7、66.7、60.0μg/mL,回收率分别为93.0%~98.5%、92.3%~94.7%、94.9%~99.2%。对市售样品进行测定,结果与国标高效液相色谱法一致,表明该法测定枣果实中葡萄糖、果糖、蔗糖含量准确可靠,且简便、快速,可为枣原料及其加工食品的质量控制提供参考。
A quantitative nuclear magnetic resonance spectroscopy(qNMR)method was established to determine the content of soluble sugar(glucose,fructose and sucrose)in jujube.The sample was extracted with deuterated water by ultrasonic,and determined by hydrogen spectroscopy using potassium hydrogen phthalate as internal standard and Zgpr as determination sequence.The results showed that the error between the measured value and the theoretical value of the linear equation slop of glucose,fructose and sucrose was less than 2%,the linear correlation coefficients were greater than 0.9999,the RSD was less than 0.5%,the limit of detection of the three sugars were 23.9μg/mL,20.0μg/mL,18.0μg/mL,limit of quantitation were 79.7μg/mL,66.7μg/mL,60.0μg/mL,the recoveries were 93.0%-98.5%,92.3%-94.7%,94.9%-99.2%respectively.The results of the determination of commercial samples were consistent with those of the national standard high performance liquid chromatography method,which showed that the qNMR method was accurate,reliable,simple and rapid,and could provide reference for the quality control of jujube and its products.
作者
杨静
何静
张衍杨
张改红
白冰
杨靖
YANG Jing;HE Jing;ZHANG Yanyang;ZHANG Gaihong;BAI Bing;YANG Jing(School of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou 450000,China;Technology Center,China Tobacco Shandong Industrial Co.,Ltd.,Qingdao 261000,China)
出处
《食品科技》
CAS
北大核心
2023年第6期281-285,共5页
Food Science and Technology
基金
河南省科技攻关项目(192102110212)。
关键词
枣
可溶性糖
定量核磁共振波谱法
jujube
soluble sugar
quantitative nuclear magnetic resonance spectroscopy
